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首页> 外文期刊>Biotechnology Progress >Comparative Evaluation of Acid and Alkaline Sulfite Pretreatments for Enzymatic Saccharification of Bagasses from Three Different Sugarcane Hybrids
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Comparative Evaluation of Acid and Alkaline Sulfite Pretreatments for Enzymatic Saccharification of Bagasses from Three Different Sugarcane Hybrids

机译：酸和碱性亚硫酸盐预处理对三种不同甘蔗杂交种的酶糖化预处理的比较评价

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摘要

Sugarcane bagasses from three experimental sugarcane hybrids and a mill-reference sample were used to compare the efficiency and mode of action of acid and alkaline sulfite pretreatment processes. Varied chemical loads and reaction temperatures were used to prepare samples with distinguished characteristics regarding xylan and lignin removals, as well as sulfonation levels of residual lignins. The pretreatment with low sulfite loads (5%) under acidic conditions (pH 2) provided maximum glucose yield of 70% during enzymatic hydrolysis with cellulases (10 FPU/g) and beta-glucosidases (20 UI/g bagasse). In this case, glucan enzymatic conversion from pretreated materials was mostly associated with extensive xylan removal (70-100%) and partial delignification occurred during the pretreatment. The use of low sulfite loads under acidic conditions required pretreatment temperatures of 160 degrees C. In contrast, at a lower pretreatment temperature (120 degrees C), alkaline sulfite process achieved similar glucan digestibility, but required a higher sulfite load (7.5%). Residual xylans from acid pretreated materials were almost completely hydrolysed by commercial enzymes, contrasting with relatively lower xylan to xylose conversions observed in alkaline pretreated samples. Efficient xylan removal during acid sulfite pretreatment and during enzymatic digestion can be useful to enhance glucan accessibility and digestibility by cellulases. Alkaline sulfite process also provided substrates with high glucan digestibility, mainly associated with delignification and sulfonation of residual lignins. The results demonstrate that temperature, pH, and sulfite can be combined for reducing lignocellulose recalcitrance and achieve similar glucan conversion rates in the alkaline and acid sulfite pretreated bagasses. (C) 2018 American Institute of Chemical Engineers
机译：采用三种实验甘蔗杂交种和研磨铝橡胶的甘蔗囊和研磨剂参考样品进行比较酸和碱性亚硫酸盐预处理方法的作用效率和模式。使用变化的化学载荷和反应温度来制备具有关于木聚糖和木质素去除的杰出特性的样品，以及残留木质素的磺化水平。在酸性条件（pH2）下具有低亚硫酸盐载荷（5％）的预处理在用纤维素酶（10fpu / g）和β-葡糖苷酶（20 ui / g bagasse）中提供了70％的最大葡萄糖产率为70％。在这种情况下，预处理材料的葡聚糖酶促转化大多数与广泛的木聚糖去除（70-100％）和预处理期间发生部分去脱矿。在酸性条件下使用低亚硫酸盐载荷的预处理温度为160℃。相比之下，在较低的预处理温度（120℃）下，碱性亚硫酸盐过程达到了相似的葡聚糖消化率，但需要更高的亚硫酸盐载荷（7.5％）。来自酸预处理材料的残留的Xylans几乎完全通过商业酶水解，与在碱预处理样品中观察到的木糖转化相对较低的木聚糖对比。高效的木聚糖去除在酸性亚硫酸盐预处理期间和酶消化期间可用于提高胰纤维素酶的葡聚糖可接近性和消化率。碱性亚硫酸盐工艺还提供了具有高葡聚糖消化率的基材，主要与残留木质素的脱氨酸和磺化有关。结果表明，温度，pH和亚硫酸盐可以组合用于减少木质纤维素克批量并达到碱性和酸性亚硝酸亚硫酸盐的类似葡聚糖转化率。 （c）2018美国化学工程研究所

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