• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Production and Characterization of Exopolysaccharides from an Enthomopathogenic Fungus Cordyceps militaris NG3
【24h】

Production and Characterization of Exopolysaccharides from an Enthomopathogenic Fungus Cordyceps militaris NG3

机译：来自血致疗法真菌冬虫夏草MILITARIS NG3的外偶糖的生产与表征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Optimization of the submerged culture conditions for the mycelial growth and production of expolysaccharide (EPS)from a newly isolated Cordyceps species (C.militaris NG3) was studied in flask cultures.The optimal temperature and initial pH POWDER (10 g/L) was the most suitabhle carbon and nitrogen source for both mycelial growth and EPS production.There was a distinguishable morphological changes in mycelium grown between organic and inorganic nitrogen sourese.A smooth jpellet growth with heavy hyphal thickness was observed in the medium containing orgaic nitrogen sources,whereas irregular pellets with less hairy region were formed in the medium containing inorganic nitrogen sources.Morehighly branched cells appearing in the medium of organic nitrogen sources seemed a favorable morphological form for both EPS production and mycelial growth Under optimal culture conditions the maximum concentrations of mycelial growth and EPS were 17.6 and 3.4 g/L in a 5-L stirred-tank fermenter.Four groups of EPSs (designated as Fr-I,Fr-II,F-III,and Fr-IV)were obtained from the culture filtates by size exclusion chromatoraphy (SEC) and their molecular characteristics were examined by a multiangle laser-light scattering(MALLS) and refractive index (RI) detector system.The weight-average molar masses of the Fr-I,Fr-II,Fr-III,and Fr-IV of EPS were determined to be 2.262 x10_6,3.348 x 10~5 1.049 x 10~5 and 5.059 x 10~4 g/mol respectively All four EPSs showed very lwo polydispersity indices ranging from 1.00 to 1.18 The Sec/MALLS analysis revealed that the molvecular shape of the Fr-I was a rigid pherer suspected to be an aggregate of complex polysaccharides,the Fr-II and Fr-III were nearly globgular in shape,and the Fr-IV was an almost rodlike structure.
机译：在烧瓶培养物中研究了从新分离的冬虫夏草种类（C.militaris Ng3）的菌丝体生长和产生蛋白质生长和产量（EPS）的优化。最佳温度和初始pH粉末（10g / L）是菌丝生长和EPS生产的大多数旅游碳和氮源。在有机和无机氮气中生长的菌丝体的可区分形态变化。在含有原子氮源的培养基中观察到具有重封螯合厚度的平滑JPELLET生长，而不规则在含有无机氮源的培养基中形成具有较少毛发区域的粒料。在有机氮源介质中出现的高压支链细胞似乎是艾克斯生产和在最佳培养条件下的菌丝产量和菌丝生长的良好形态形式，最大浓度的菌丝生长和EPS在5-L搅拌罐发酵罐中是17.6和3.4 g / l.four gr通过尺寸排除色谱（SEC）从培养物中获得EPSS（指定为FR-I，FR-II，F-III和FR-IV），并通过多聚激光散射检查它们的分子特性。商场）和折射率（RI）探测器系统。FR-I，FR-II，FR-III和EPS的FR-IV的重量平均摩尔质量为2.262 x10_6,3.348 x 10〜5 1.049 x 10〜5和5.059 x 10〜4 g / mol分别显示所有四个eps都显示出极限的多分散性指数从1.00到1.18的秒为1.18，秒/商场分析表明，FR-i的摩尔普形状是一个刚性钳子，令人耳机复合多糖的聚集体，FR-II和FR-III几乎是球形的形状，FR-IV几乎是杆状结构。

著录项

  • 来源
    《Biotechnology Progress》 |2003年第2期|共8页
  • 作者

    Sang Woo Kim; Chun Ping Xu; Hye Jin Hwang; Jang Won Choi; Chan Woo Kim;

  • 作者单位

    Department of Biotechnology Department of Natural Resources and Center for Laboratory Facilities Daegu University Kyungasn Kyungbuk 712-714 Korea;

    Department of Biotechnology Department of Natural Resources and Center for Laboratory Facilities Daegu University Kyungasn Kyungbuk 712-714 Korea;

    Department of Biotechnology Department of Natural Resources and Center for Laboratory Facilities Daegu University Kyungasn Kyungbuk 712-714 Korea;

    Department of Biotechnology Department of Natural Resources and Center for Laboratory Facilities Daegu University Kyungasn Kyungbuk 712-714 Korea;

    Department of Biotechnology Department of Natural Resources and Center for Laboratory Facilities Daegu University Kyungasn Kyungbuk 712-714 Korea;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物工程学（生物技术）;分子生物学;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号