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Derivation and identification of a mechanistic model for a branched enzyme‐catalyzed carboligation

机译：支化酶催化胶结性机械模型的衍生与识别

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Abstract > The kinetic description of enzyme‐catalyzed reactions is a core task in biotechnology and biochemical engineering. In particular, mechanistic kinetic models help from the discovery of the biocatalyst throughout its application. Chemo‐ or enantioselective enzyme reactions often undergo two alternative pathways for the release of two different products from a central intermediate. For these types of reaction, no explicit reaction equations have been derived so far. To this end, we extend the commonly used Cleland's notation for branched reaction pathways and explicitly derive the rate expressions for two‐coupled ordered bi–uni reactions. This mechanism also leads to a ping‐pong bi–bi mechanism for a transfer reaction between the two products via the same central intermediate of the reaction system. Using the cross‐ligation of benzaldehyde and propanal catalyzed by the thiamine diphosphate‐dependent enzyme benzaldehyde lyase from Pseudomonas fluorescens yielding ( R )‐2‐hydroxy‐1‐phenylbutan‐1‐one as a case study, we performed model‐based experimental analysis to show that such a reaction mechanism can be modeled mechanistically and leads to reasonable kinetic parameters. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 酶催化反应的动力学描述是生物技术和生物化学工程中的核心任务。特别是，机械动力学模型从整个应用中发现生物催化剂的发现。化学或对映选择性酶反应通常经过两种替代途径，用于释放来自中间中间体的两种不同产品。对于这些类型的反应，到目前为止没有产生明确的反应方程。为此，我们扩展了常用的克兰德兰德对分支反应途径的符号，并明确地推导出双耦合有序双UNI反应的速率表达。该机制还导致Ping Pong Bi-Bi机制通过相同的反应系统的中间体中间体在两种产品之间转移反应。利用硫胺素二磷酸二磷酸依赖性酶苯甲醛裂解酶催化的苯甲醛和丙醛的交叉连接假单胞菌荧光荧光素</ i> 屈服（ R </ I> ）-2-羟基-1-苯基丁丹-1-1作为一种情况，我们进行了基于模型的实验分析，表明这种反应机制可以机械地建模并导致合理的动力学参数。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15005/'>《Biotechnology Progress》</a> <b style="margin: 0 2px;">|</b><span>2019年第6期</span><b style="margin: 0 2px;">|</b><span>共13页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ohs Rüdiger&option=202" target="_blank" rel="nofollow">Ohs Rüdiger;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fischer Konrad&option=202" target="_blank" rel="nofollow">Fischer Konrad;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sch?pping Marie&option=202" target="_blank" rel="nofollow">Sch?pping Marie;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Spiess Antje C.&option=202" target="_blank" rel="nofollow">Spiess Antje C.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Aachener Verfahrenstechnik—Enzyme Process TechnologyRWTH Aachen UniversityAachen Germany;</p> <p>Aachener Verfahrenstechnik—Enzyme Process TechnologyRWTH Aachen UniversityAachen Germany;</p> <p>Aachener Verfahrenstechnik—Enzyme Process TechnologyRWTH Aachen UniversityAachen Germany;</p> <p>Aachener Verfahrenstechnik—Enzyme Process TechnologyRWTH Aachen UniversityAachen Germany;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/15.html" title="生物科学">生物科学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=branched reaction networks&option=203" rel="nofollow">branched reaction networks;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cross‐carboligation&option=203" rel="nofollow">cross‐carboligation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=kinetic modeling&option=203" rel="nofollow">kinetic modeling;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=parameter estimation&option=203" rel="nofollow">parameter estimation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ThDP‐dependent enzymes&option=203" rel="nofollow">ThDP‐dependent enzymes;</a> </p> <div class="translation"> 机译：分支反应网络;交叉粘土;动力学建模;参数估计;依赖于依赖于THDP依赖性酶; 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