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首页> 外文期刊>Cytokine >Murine abortion is associated with enhanced interleukin-6 levels at the feto-maternal interface.
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Murine abortion is associated with enhanced interleukin-6 levels at the feto-maternal interface.

机译：小鼠流产与胎儿-母亲界面的白细胞介素6水平升高有关。

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CBA/JXDBA/2J murine abortion is known to be associated with increased local and peripheral Th1-cytokines levels. The role of the pro-inflammatory interleukin-6 (IL-6) in murine abortion remains unclear. In humans, IL-6 was reported to be elevated at the onset of spontaneous abortion. The aim of our study was to evaluate the levels of IL-6 during murine pregnancy in (1) the normal murine pregnancy combination CBA/JXBALB/c and in (2) the CBA/JXDBA/2J abortion prone mating combination. We measured IL-6 serum levels by ELISA and local (placental and decidual) IL-6 levels by flow cytometry and immunohistochemistry. The expression of the IL-6 receptor gp80 was further analyzed. We additionally evaluated the number of mast cells and macrophages at the feto-maternal interface as a putative IL-6 source in reproductive tissues. IL-6 and gp80 were expressed in decidual cells as well as in different trophoblast types. Flow cytometry analysis showed increased numbers of IL-6+ cells in abortion placentas and deciduas compared to control pregnant mice. We observed an elevated number of mast cells and macrophages at the feto-maternal interface from abortion mice in comparison to control mice. Interestingly, we found very high numbers of mast cells, macrophages and IL-6+ cells in resorption tissue compared to control tissues. Flow cytometry studies confirmed that macrophages are being an important source of IL-6 at the feto-maternal interface. The mRNA IL-6 levels were also enhanced in placenta and decidua from mice with high abortion rate compared to normal pregnant mice, as analyzed by RT-PCR. Our results suggest that IL-6 produced not only by immunocompetent cells such as macrophages and mast cells, but also by trophoblasts and decidua cells, is directly involved in the pathology of abortion.
机译：已知CBA / JXDBA / 2J小鼠流产与局部和外周Th1细胞因子水平升高有关。促炎性白介素6（IL-6）在小鼠流产中的作用仍不清楚。在人类中，据报道自发流产时IL-6升高。我们研究的目的是评估（1）正常鼠妊娠组合CBA / JXBALB / c和（2）易流产CBA / JXDBA / 2J组合在鼠妊娠中的IL-6水平。我们通过ELISA测量了IL-6血清水平，并通过流式细胞术和免疫组化测量了局部（胎盘和蜕膜）IL-6水平。进一步分析了IL-6受体gp80的表达。我们还评估了胎儿-母体界面肥大细胞和巨噬细胞的数量，作为生殖组织中公认的IL-6来源。 IL-6和gp80在蜕膜细胞以及不同的滋养细胞类型中表达。流式细胞仪分析显示，与对照妊娠小鼠相比，流产胎盘和蜕皮中IL-6 +细胞数量增加。我们观察到与对照小鼠相比，流产小鼠的胎儿-母体界面肥大细胞和巨噬细胞数量增加。有趣的是，与对照组织相比，我们在吸收组织中发现了大量的肥大细胞，巨噬细胞和IL-6 +细胞。流式细胞术研究证实，巨噬细胞是胎儿-母亲界面中IL-6的重要来源。通过RT-PCR分析，与正常妊娠小鼠相比，流产率高的小鼠的胎盘和蜕膜中的mRNA IL-6水平也提高了。我们的结果表明，IL-6不仅由具有免疫功能的细胞（例如巨噬细胞和肥大细胞）产生，而且由滋养细胞和蜕膜细胞产生，直接与流产病理有关。

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