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首页> 外文期刊>Biotechnology and Bioengineering >Fabrication of a biomimetic spinal cord tissue construct with heterogenous mechanical properties using intrascaffold cell assembly
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Fabrication of a biomimetic spinal cord tissue construct with heterogenous mechanical properties using intrascaffold cell assembly

机译：使用脚手架芯片组件制备具有异质机械性能的生物仿真脊髓组织构建体

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摘要

In tissue engineering studies, scaffolds play a very important role in offering both physical and chemical cues for cell growth and tissue regeneration. However, in some cases, tissue regeneration requires scaffolds with high mechanical properties (e.g., bone and cartilage), while cells need a soft mechanical microenvironment. In this study, to mimic the heterogenous mechanical properties of a spinal cord tissue, a biomimetic rat tissue construct is fabricated. A collagen-coated poly(lactic-co-glycolic acid) scaffold is manufactured using thermally induced phase separation casting. Primary rat neural cells (P01 Wistar rat cortex) with soft hydrogels are later printed within the scaffold using an image-guided intrascaffold cell assembly technique. The scaffolds have unidirectional microporous structure with parallel axial macrochannels (260 +/- 4 mu m in diameter). Scaffolds showed mechanical properties similar to rat spine (ultimate tensile strength: 0.085 MPa, Young's modulus [stretch]: 0.31 MPa). The bioink composed of gelatin/alginate/fibrinogen is precisely printed into the macrochannels and showed mechanical properties suitable for neural cells (Young's modulus [compressive]: 3.814 kPa). Scaffold interface, cell viability, and immunostaining analyses show uniform distribution of stable, healthy, and elongated neural cells and neurites over 14 culture days in vitro. The results demonstrated that this method can serve as a valuable tool to aid manufacturing of tissue constructs requiring heterogenous mechanical properties for complex cell and/or biomolecule assembly.
机译：在组织工程研究中，脚手架在为细胞生长和组织再生提供了一种物理和化学提示方面发挥着非常重要的作用。然而，在某些情况下，组织再生需要具有高机械性能的支架（例如，骨骼和软骨），而细胞需要软机械微环境。在该研究中，为了模拟脊髓组织的异质力学性能，制造了一种仿生大鼠组织构建体。使用热诱导的相分离浇铸制造胶原涂覆的聚（乳酸 - 共乙醇酸）支架。用软水凝胶的原代大鼠神经细胞（P01 Wistar大鼠皮层）使用图像引导的内含物细胞组装技术后来印刷在支架内。支架具有单向微孔结构，具有平行轴向宏观通道（直径260 +/-4μm）。支架显示出类似于大鼠脊柱的机械性能（极限拉伸强度：0.085MPa，杨氏模量[拉伸]：0.31MPa）。由明胶/藻酸盐/纤维蛋白原组成的生物链精确地印刷到宏观通道中，并显示出适合神经细胞的机械性能（杨氏模量[压缩]：3.814kPa）。支架界面，细胞活力和免疫染色分析显示出稳定，健康和细长的神经细胞和体外14个培养日内的神经肌腱均匀分布。结果表明，该方法可以作为有价值的工具，以帮助制造需要对复杂的细胞和/或生物分子组件的异质机械性能的组织构建体的制造。

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