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首页> 外文期刊>Cytokine >Expression of IL-17 in human memory CD45RO+ T lymphocytes and its regulation by protein kinase A pathway.
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Expression of IL-17 in human memory CD45RO+ T lymphocytes and its regulation by protein kinase A pathway.

机译：IL-17在人记忆CD45RO + T淋巴细胞中的表达及其蛋白激酶A途径的调控。

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In the present study, the authors compared the interleukin 17 (IL-17 expression of human naive and phenotypically defined memory T cells as well as its regulation by cAMP pathway. Our data showed that IL-17 mRNA was highly expressed in memory human peripheral CD8(+)45RO+T cells and CD4(+)45RO+T cells when peripheral blood mononuclear cells were first stimulated with ionomycin/PMA. IL-17 expression in memory CD8(+)T cells required accessory signals since culture of ionomycin/PMA-activated CD8(+)45RO+T cells alone did not result to IL-17 expression. In contrast, memory CD4(+)T cell population seems to be more independent. IL-17 and interferon gamma(IFN-gamma) mRNA were both inhibited in the presence of PGE2 or the cAMP analogue (dibutyryl-cAMP), while the anti-inflammatory cytokine IL-10 was highly increased. In contrast, naive CD45RA+T cells were unable to express IL-17 whatever the culture conditions. Naive CD4(+)and CD8(+)T cells were sensitive to the PKA regulatory pathway since they represent a significant source of IL-10 when PBMC were first cultured with ionomycin/PMA in the presence of either PGE2 or db-cAMP. The authors showed that naive cells are highly dependent to their microenvironment, since culture of ionomycin/PMA-activated CD45RA+T cells alone did not result in detectable levels of cytokines even in the presence of PGE2. Results also showed that PGE2 induced quite the same levels of intracellular cAMP in naive and memory cells suggesting that these cell populations are equally sensitive to PGE2. However, we suggest that PGE2 may be more efficient in blocking both IL-17 and IFN-gamma expression in already primed memory T cells, rather than in suppressing naive T cells that could represent a significant source of IL-10. Data suggest that PKA activation pathway plays a critical role in the regulation of cytokine profiles and consequently the functional properties of both human naive and memory CD4(+) and CD8(+)T cells during the immune and inflammatory processes. Copyright 1999 Academic Press.
机译：在本研究中，作者比较了白细胞介素17（IL-17在人类幼稚和表型定义的记忆T细胞中的表达及其通过cAMP途径的调控。我们的数据显示，IL-17 mRNA在记忆人类外周血CD8中高表达。首次用离子霉素/ PMA刺激外周血单个核细胞时，（+）45RO + T细胞和CD4（+）45RO + T细胞。由于离子霉素/ PMA的培养，记忆CD8（+）T细胞中的IL-17表达需要辅助信号活化的CD8（+）45RO + T细胞不会单独导致IL-17表达，相反，记忆CD4（+）T细胞群体似乎更独立，IL-17和干扰素γ（IFN-γ）mRNA在存在PGE2或cAMP类似物（二丁酰-cAMP）的情况下，它们都被抑制，而抗炎细胞因子IL-10则大大增加，相比之下，无论培养条件如何，未成熟的CD45RA + T细胞均不能表达IL-17。幼稚的CD4（+）和CD8（+）T细胞对PKA调节途径敏感，因为它们当PBMC在PGE2或db-cAMP存在下首次与离子霉素/ PMA培养时，代表IL-10的重要来源。这组作者表明，天真细胞高度依赖于其微环境，因为单独培养离子霉素/ PMA激活的CD45RA + T细胞即使在存在PGE2的情况下也无法导致可检测水平的细胞因子。结果还表明，PGE2在幼稚和记忆细胞中诱导了相同水平的细胞内cAMP，这表明这些细胞群对PGE2同样敏感。但是，我们建议PGE2可能在阻断已经灌注的记忆T细胞中更有效地阻断IL-17和IFN-γ的表达，而不是抑制可能代表IL-10重要来源的幼稚T细胞。数据表明，PKA激活途径在调节细胞因子的过程中起着至关重要的作用，因此在免疫和炎症过程中，人类幼稚的和记忆的CD4（+）和CD8（+）T细胞的功能特性。版权所有1999，学术出版社。

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