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首页> 外文期刊>Cytokine >Role of cytokine-induced neutrophil chemoattractant-2 (CINC-2) alpha in a rat model of chronic bronchopulmonary infections with Pseudomonas aeruginosa.
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Role of cytokine-induced neutrophil chemoattractant-2 (CINC-2) alpha in a rat model of chronic bronchopulmonary infections with Pseudomonas aeruginosa.

机译：细胞因子诱导的中性粒细胞趋化因子2（CINC-2）α在铜绿假单胞菌慢性支气管肺部感染大鼠模型中的作用。

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In order to investigate the role of the cytokine-induced neutrophil chemoattractant (CINC) in chronic bronchopulmonary infection, we developed a rat model of bronchopulmonary infection with Pseudomonas aeruginosa by using the agar bead method, and determined the kinetics of bacterial and cell number, as well as the concentrations of CINC-1, CINC-2, and CINC-3 in bronchoalveolar lavage (BAL) fluids in this model. The bacterial number in the lung rapidly increased from days 1 to 4, and declined 14 days after challenge. Neutrophil number in BAL fluid increased up to one day after challenge, and then slowly decreased during 14 days post-challenge. Among the CINCs, the local production of CINC-2 alpha sharply increased at day 1 and then decreased until day 4 post-challenge, while the local production of CINC-1 slightly increased at day 1 post-challenge. Neither CINC-2 beta nor CINC-3 were detected during the entire course of the infection. Increased CINC-2 mRNA expression in the lung tissue after challenge was associated with CINC-2 alpha production in BAL fluid. Moreover, an immunohistochemical study demonstrated the localization of CINC-1 and CINC-2 alpha primarily in alveolar macrophages and, to a much lesser extent, in bronchial epithelium of infected lung tissues, whereas CINC-2 beta and CINC-3 were not detected. When anti-CINC-1 or anti-CINC-2 alpha polyclonal antibodies were used for neutralizing neutrophil chemotactic activities in BAL fluids, the anti-CINC-2 alpha antibody inhibited 70% of the chemotactic activity in BAL fluids from infected rats at day 1 after challenge. No inhibition was observed by anti-CINC-1 antibody. These data indicate that CINC-2 alpha, which is produced by alveolar macrophages and bronchial epithelial cells, plays a pivotal role in neutrophil accumulation in the airway of a rat model of chronic bronchopulmonary infection with P. aeruginosa. Copyright 2000 Academic Press.
机译：为了研究细胞因子诱导的中性粒细胞趋化因子（CINC）在慢性支气管肺部感染中的作用，我们通过琼脂珠法建立了铜绿假单胞菌感染的支气管肺部感染大鼠模型，并确定了细菌和细胞数的动力学，如下以及该模型中支气管肺泡灌洗液（BAL）中CINC-1，CINC-2和CINC-3的浓度。攻击后第14天，肺中的细菌数量迅速增加，从第1天到第4天下降。 BAL液中的嗜中性粒细胞数在攻击后的一天内增加，然后在攻击后14天内缓慢下降。在CINC中，CINC-2 alpha的本地产量在攻击后第1天急剧增加，然后下降到第4天，而CINC-1的本地产量在攻击后第1天略有增加。在整个感染过程中均未检测到CINC-2 beta和CINC-3。攻击后肺组织中CINC-2 mRNA表达的增加与BAL液中CINC-2α的产生有关。此外，一项免疫组织化学研究表明，CINC-1和CINC-2 alpha主要位于肺泡巨噬细胞中，而在较小的程度上位于受感染肺组织的支气管上皮中，而未检测到CINC-2 beta和CINC-3。当使用抗CINC-1或抗CINC-2α多克隆抗体中和BAL液中嗜中性粒细胞的趋化活性时，抗CINC-2α抗体在第1天抑制了感染大鼠BAL液中70％的趋化活性。挑战后。抗CINC-1抗体未观察到抑制作用。这些数据表明，由肺泡巨噬细胞和支气管上皮细胞产生的CINC-2α在铜绿假单胞菌慢性大鼠支气管感染大鼠模型的气道中对嗜中性粒细胞积累起关键作用。版权所有2000学术出版社。

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