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首页> 外文期刊>Cytokine >IL-4 and IL-13 mRNA real-time PCR quantification on whole blood to assess allergic response.
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IL-4 and IL-13 mRNA real-time PCR quantification on whole blood to assess allergic response.

机译：对全血进行IL-4和IL-13 mRNA实时PCR定量以评估过敏反应。

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Although routinely used in clinical practice, skin prick tests and serum specific IgE often fail to distinguish between IgE-sensitization and symptomatic IgE-mediated allergy. There is therefore a need for new laboratory tests relating allergic symptoms to the offending agent. In this way, we evaluated the diagnostic reliability of a new whole blood quantitative real-time PCR assay for IL-4 and IL-13 mRNAs. We compared the response of cat allergic patients and non-cat allergic controls upon anti-IgE and cat allergen (Fel d1) stimulation of whole blood. Allergen addition led to a significant increase of IL-4 and IL-13 mRNAs in allergic patients compared to non-allergic controls (p<0.0001). Both cytokine mRNA levels were strongly correlated and peaked within 2 h after Fel d1 or anti-IgE addition. This rapid increase as well as purification experiments led us to the conclusion that basophils represent an important if not the main source of both transcripts in this setting. The effect was allergen-specificsince not observed when stimulating blood from cat allergic patients with birch pollen. Interestingly, we found that IFN-gamma mRNA, contrary to IL-4 mRNA, reached higher levels in response to Fel d1 with control individuals than with patients allergic to cat. This study shows that whole blood real-time PCR is a valuable method for IL-4 and IL-13 mRNA measurement after in vitro allergen challenge. We suggest that it might be useful, complementing conventional markers, for the diagnosis and the follow-up of allergic diseases. Further assessments are required to evaluate the clinical potential of this technique.
机译：尽管通常在临床实践中使用，但皮肤点刺试验和血清特异性IgE常常无法区分IgE致敏和症状性IgE介导的过敏。因此，需要新的将过敏症状与违法行为相关的实验室检查。通过这种方式，我们评估了针对IL-4和IL-13 mRNA的新型全血实时定量PCR检测方法的诊断可靠性。我们比较了猫过敏患者和非猫过敏对照对全血抗IgE和猫过敏原（Fel d1）的反应。与非过敏对照组相比，添加过敏原导致过敏患者的IL-4和IL-13 mRNA显着增加（p <0.0001）。两种细胞因子mRNA水平均密切相关，并在添加Fel d1或抗IgE后2小时内达到峰值。这种快速增长以及纯化实验使我们得出结论：在这种情况下，嗜碱性粒细胞代表了两种转录本的重要来源，即使不是主要来源。从刺激桦树花粉的猫变态反应患者的血液中未观察到该效应是变应原特异性的。有趣的是，我们发现与IL-4 mRNA相反，IFN-γmRNA在控制个体中对Fel d1的反应比对猫过敏的患者达到更高的水平。这项研究表明，全血实时PCR是体外过敏原激发后测量IL-4和IL-13 mRNA的有价值的方法。我们建议，作为常规标志物的补充，对于过敏性疾病的诊断和随访可能有用。需要进一步评估以评估该技术的临床潜力。

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