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首页> 外文期刊>Cytokine >Porphyromonas gingivalis antagonises Campylobacter rectus induced cytokine production by human monocytes.
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Porphyromonas gingivalis antagonises Campylobacter rectus induced cytokine production by human monocytes.

机译：牙龈卟啉单胞菌拮抗直弯弯曲菌诱导的人单核细胞产生的细胞因子。

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摘要

Porphyromonas gingivalis and Campylobacter rectus are two major bacterial species implicated in the pathogenesis of periodontitis. P. gingivalis can antagonise the inflammatory response to other periodontal pathogens, a property commonly attributed to its lipopolysaccharide (LPS). The aim of this study was to investigate the capacity of P. gingivalis to antagonise C. rectus induced cytokine stimulation from human monocytes, and to investigate the involvement of its LPS. Primary human monocytes and Monomac-6 cells were challenged with culture supernatants from P. gingivalis and C. rectus, and levels of IL-1beta, IL-6 and IL-8 produced were measured by ELISA after 6h incubation. Purified P. gingivalis LPS was also added alone or in combination with C. rectus culture supernatant. Both species significantly stimulated the production of all three cytokines from the two cell lines, but P. gingivalis was considerably weaker inducer. Co-stimulation of the cells with P. gingivalis and C. rectus suppressed the cytokine-stimulatory capacity of the latter. P. gingivalis LPS alone was sufficient to antagonise IL-6 and IL-8, but not IL-1beta stimulation by C. rectus. In conclusion, mixed infections may impair host immune responses by reducing pro-inflammatory cytokine levels, which may be of relevance to the pathogenesis of periodontitis.
机译：牙龈卟啉单胞菌和直肌弯曲杆菌是涉及牙周炎发病机理的两种主要细菌。牙龈卟啉单胞菌可以拮抗对其他牙周病原体的炎症反应，这通常归因于其脂多糖（LPS）。这项研究的目的是调查牙龈卟啉单胞菌拮抗直肌引起的人单核细胞刺激细胞因子的能力，并研究其脂多糖的参与。用牙龈卟啉单胞菌和直肌弯曲杆菌的培养上清液攻击原代人单核细胞和Monomac-6细胞，温育6小时后通过ELISA测定产生的IL-1beta，IL-6和IL-8的水平。纯化的牙龈卟啉单胞菌LPS也可单独添加或与直肌弯曲杆菌培养上清液一起添加。两种物种均显着刺激了两种细胞系中所有三种细胞因子的产生，但牙龈卟啉单胞菌的诱导剂弱得多。与牙龈卟啉单胞菌和直肌念珠菌共同刺激细胞抑制了后者的细胞因子刺激能力。单独的牙龈卟啉单胞菌LPS足以拮抗直肌梭菌刺激IL-6和IL-8，但不能拮抗IL-1beta。总之，混合感染可通过降低促炎细胞因子水平来削弱宿主的免疫反应，这可能与牙周炎的发病机理有关。

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