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首页> 外文期刊>Cytokine >Quantitative comparison of cytokine mRNA and inflammatory responses in cutaneous late phase allergic reactions.
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Quantitative comparison of cytokine mRNA and inflammatory responses in cutaneous late phase allergic reactions.

机译：皮肤晚期过敏反应中细胞因子mRNA和炎症反应的定量比较。

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The aim of this study was to quantitatively compare expression of mRNA for IL-5 and IFN-gamma with the frequency of mRNA-positive cells, total and activated eosinophils, neutrophils, lymphocytes, and vessels expressing adhesion molecules. Replicate biopsies of skin LPR to pollen antigens (Ag) and control injection sites (B) at 6 and 24 h were assessed for: (1) mRNA for IL-5 and IFN-gamma by quantitative RT-PCR (QC-RT/PCR); (2) frequency of cells expressing mRNA for IL-5 and IFN-gamma by in situ hybridization (ISH); (3) inflammatory cells and adhesion molecule expression. More mRNA for IL-5 was found in Ag- than in B-injected sites at 6 and 24 h by both QC-RT/PCR and ISH. Small amounts of mRNA for IFN-gamma were detected in Ag sites by QC-RT/PCR at 6 and 24 h, but were not significantly different than at B sites. The frequency of IFN-gamma mRNA(+)cells was higher in Ag than in B sites at 6 h. There was no correlation between the amount if IL-5 detected by QC-RT/PCR and frequency of IL-5 mRNA(+)cells by ISH. These findings also did not correlate with the degree of inflammatory responses. In conclusion: (1) greater IL-5 than IFN-gamma deposition in Ag sites suggests Th(2)predominance in LPR; (2) lack of correlation between QC-RT/PCR and ISH findings may reflect varying mRNA content of inflammatory cells. Copyright 2000 Academic Press.
机译：这项研究的目的是定量比较IL-5和IFN-γ的mRNA表达与mRNA阳性细胞，总嗜酸性粒细胞和活化嗜酸性粒细胞，嗜中性粒细胞，淋巴细胞以及表达粘附分子的血管的频率。评估在第6和24小时将皮肤LPR复制为花粉抗原（Ag）和对照注射部位（B）的活检组织：（1）通过定量RT-PCR（QC-RT / PCR）检测IL-5和IFN-γ的mRNA ）; （2）通过原位杂交（ISH）表达IL-5和IFN-γ的mRNA的细胞的频率; （3）炎性细胞与黏附分子的表达。通过QC-RT / PCR和ISH，在6和24 h时，在Ag注射的部位比在B注射的部位发现了更多的IL-5 mRNA。通过QC-RT / PCR在6和24 h在Ag位点检测到少量的IFN-γmRNA，但与B位点没有显着差异。在6小时时，Ag中的IFN-γmRNA（+）细胞的频率高于B点。 QC-RT / PCR检测到IL-5的量与ISH检测IL-5 mRNA（+）细胞的频率之间没有相关性。这些发现也与炎症反应的程度无关。结论：（1）在Ag位点，IL-5比IFN-γ沉积更大，表明Th（2）在LPR中占优势; （2）QC-RT / PCR与ISH结果之间缺乏相关性，可能反映了炎症细胞中mRNA含量的变化。版权所有2000学术出版社。

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