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首页> 外文期刊>Cytokine >Tumour necrosis factor-alpha up-regulates the expression of BMP-4 mRNA but inhibits chondrogenesis in mouse clonal chondrogenic EC cells, ATDC5.
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Tumour necrosis factor-alpha up-regulates the expression of BMP-4 mRNA but inhibits chondrogenesis in mouse clonal chondrogenic EC cells, ATDC5.

机译：肿瘤坏死因子-α上调BMP-4 mRNA的表达，但抑制小鼠克隆软骨生成EC细胞ATDC5中的软骨生成。

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摘要

Tumour necrosis factor (TNF)-alpha causes the degradation of articular cartilage in arthritis via direct actions on chondrocytes. However, it remains unknown whether TNF-alpha affects chondrogenesis in chondroprogenitors. In the present study, we assessed the effects of TNF-alpha in vitro on chondrogenesis using mouse clonal chondrogenic EC cells, ATDC5. TNF-alpha (10 ng/ml) stimulated [3H] thymidine incorporation in undifferentiated ATDC5 cells, and suppressed cartilaginous nodule formation and the accumulation of cartilage-specific proteoglycan. We recently showed that undifferentiated ATDC5 cells express BMP-4 and that exogenously administered BMP-4 promotes chondrogenesis in these cells. Interestingly, TNF-alpha up-regulated the expression of BMP-4 mRNA in undifferentiated ATDC5 cells in time- and dose-dependent manners. However, exogenously administered BMP-4 was not capable of reversing the inhibitory action of TNF-alpha on chondrogenesis in ATDC5 cells. These results indicate that TNF-alpha stimulates both cell proliferation and BMP-4 expression but inhibits chondrogenesis in chondroprogenitor-like ATDC5 cells.
机译：肿瘤坏死因子（TNF）-α通过直接作用于软骨细胞而导致关节炎中的关节软骨退化。但是，尚不清楚TNF-α是否会影响软骨生成原的软骨形成。在本研究中，我们使用小鼠克隆软骨生成EC细胞ATDC5评估了TNF-α在体外对软骨形成的影响。 TNF-α（10 ng / ml）刺激未分化的ATDC5细胞中[3H]胸腺嘧啶核苷的掺入，并抑制软骨结节的形成和软骨特异性蛋白聚糖的积累。我们最近发现未分化的ATDC5细胞表达BMP-4，而外用BMP-4促进这些细胞中的软骨形成。有趣的是，TNF-α以时间和剂量依赖性方式上调了未分化ATDC5细胞中BMP-4 mRNA的表达。但是，外源给药的BMP-4不能逆转TNF-α对ATDC5细胞软骨形成的抑制作用。这些结果表明，TNF-α既刺激细胞增殖，又刺激BMP-4表达，但抑制软骨生成素样ATDC5细胞的软骨生成。

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