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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Simultaneous determination of baicalin, oroxylin A-7-O-glucuronide and wogonoside in rat plasma by UPLC-DAD and its application in pharmacokinetics of pure baicalin, Radix Scutellariae and Yinhuang granule
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Simultaneous determination of baicalin, oroxylin A-7-O-glucuronide and wogonoside in rat plasma by UPLC-DAD and its application in pharmacokinetics of pure baicalin, Radix Scutellariae and Yinhuang granule

机译：UPLC-DAD同时测定大鼠血浆大鼠血浆中的黄芩苷，奥氧化丁蛋白A-7-O-葡糖醛糖苷和WOGONONIDS的纯芩疫苗药代动力学，云甘蓝菌和银黄颗粒的应用

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摘要

A novel UPLC-DAD method was developed and validated for the simultaneous determination of baicalin (baicalein-7-glucuronide, BG), oroxylin A-7-O-glucuronide (OAG) and wogonoside (WG) in rat plasma using rutin as the internal standard. Plasma samples were precipitated using acetonitrile containing 0.1% formic acid. Separation was performed on an Agilent Eclipse Plus C-18 column (2.1 x 50 mm, 1.8 mu m) using gradient acetonitrile and 0.2% formic acid water solution as mobile phase. The flow-rate was set at 0.4 mL/min and the eluate was detected at 275 nm. The method was linear over the ranges of 0.075-17.50, 0.050-12.60 and 0.056-14.10 mu g/mL for BG, OAG and WG, respectively. The intra- and inter-day precisions were respectively <4.8% and 6.4%. All of the limits of detection of three analytes in rat plasma were 0.01 mu g/mL, whereas the limits of quantification were, respectively, 0.035, 0.025 and, 0.025 mu g/mL. This assay has been successfully applied to pharmacokinetics of BG, OAG and WG in rats after oral administration of Yinhuang granule (YHG) and comparative pharmacokinetics of BG in rats following oral administration of the pure BG, Radix Scutellariae (RS) or YHG. We speculate that some co-existing ingredients in RS or YHG may increase the absorption and elimination of BG in rat. This work may be helpful for the quality control of Yinhuang granule. Copyright (c) 2015 John Wiley & Sons, Ltd.
机译：开发了一种新型UPLC-DAD方法并验证了使用芦丁作为内部大鼠等离子体同时测定黄芩苷（Baicalin-7-葡萄糖醛酸酯，BG），oroxylina-7-O-葡萄糖醛酸酯（OAG）和Wogonoside（WG）标准。使用含0.1％甲酸的乙腈沉淀血浆样品。使用梯度乙腈和0.2％甲酸水溶液作为流动相，在安置Eclipse加上C-18柱（2.1×50mm，1.8μm）上进行分离。将流速设定为0.4ml / min，并在275nm处检测洗脱液。该方法分别为0.075-17.50,0.050-12.60和0.056-14.10μg/ ml的BG，OAG和WG的线性。内部和日间诊断分别<4.8％和6.4％。大鼠等离子体中的三种分析物的所有限制为0.01μmg/ ml，而定量的极限分别为0.035,0.025和0.025μg/ ml。该测定在口服施用纯BG后的大鼠中的大鼠口服施用后，在大鼠中成功地应用于BG，OAG和WG的药代动力学，并在口服施用纯BG，纯化的BG，radixcutellariae（Rs）或Yhg。我们推测RS或YHG中的一些共存成分可能会增加大鼠BG的吸收和消除。这项工作可能有助于银黄颗粒的质量控制。版权所有（c）2015 John Wiley＆Sons，Ltd。

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