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首页> 外文期刊>Cytokine >Adiponectin deficiency modulates adhesion molecules expression and cytokine production but does not affect disease severity in the transfer model of colitis.
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Adiponectin deficiency modulates adhesion molecules expression and cytokine production but does not affect disease severity in the transfer model of colitis.

机译：脂联素缺乏调节结肠分子转移模型中粘附分子的表达和细胞因子的产生，但不影响疾病的严重程度。

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摘要

We investigated the effect of adiponectin (APN) deficiency in the CD4(+)CD45RB(high) transfer model of colitis. Recombination activating gene (Rag)-1 knockout (KO) and Rag-1 APN KO mice receiving CD4(+)CD45RB(high) cells developed colitis of comparable severity. Colonic mRNA expression of IL-6 and IL-17 was lower in Rag-1 APN KO mice compared to Rag-1 KO mice. Rag-1 APN KO and Rag-1 KO mice released comparable amounts of IL-6 from colon cultures, whereas release of IL-17 was higher in Rag-1 APN KO compared to Rag-1 KO mice. Expression of TNFalpha mRNA was comparable in Rag-1 KO and Rag-1 APN KO mice, but protein release was lower in Rag-1 APN KO mice compared to Rag-1 KO mice. Levels of IFNgamma and IL-10 at mRNA and protein were comparable in Rag-1 KO and Rag-1 APN KO mice. Higher mRNA expression of VCAM-1 was observed in the colon of healthy APN KO compared to WT mice, while induction of colitis resulted in a comparable increase in VCAM-1 expression in Rag-1 KO and Rag-1 APN KO mice. In conclusion, although APN regulates expression of cytokines and adhesion molecules in the colon, this does not result in alteration of overall colitis severity in the CD4(+)CD45RB(high) transfer model.
机译：我们调查了结肠炎的CD4（+）CD45RB（high）转移模型中脂联素（APN）缺乏的影响。重组激活基因（Rag）-1基因敲除（KO）和Rag-1 APN KO小鼠接受CD4（+）CD45RB（high）细胞发展为可比严重程度的结肠炎。与Rag-1 KO小鼠相比，Rag-1 APN KO小鼠的IL-6和IL-17结肠mRNA表达较低。 Rag-1 APN KO和Rag-1 KO小鼠从结肠培养物中释放了相当量的IL-6，而与Rag-1 KO小鼠相比，Rag-1 APN KO中IL-17的释放更高。与Rag-1 KO小鼠相比，Rag-1 KO和Rag-1 APN KO小鼠中TNFalpha mRNA的表达相当，但Rag-1 APN KO小鼠中的蛋白质释放较低。在Rag-1 KO和Rag-1 APN KO小鼠中，mRNA和蛋白的IFNgamma和IL-10水平相当。与野生型小鼠相比，在健康的APN KO结肠中观察到更高的VCAM-1 mRNA表达，而结肠炎的诱导导致Rag-1 KO和Rag-1 APN KO小鼠中VCAM-1表达增加。总之，尽管APN调节结肠中细胞因子和粘附分子的表达，但这不会导致CD4（+）CD45RB（high）转移模型中总体结肠炎严重程度的改变。

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