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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Use of high-pH (basic/alkaline) mobile phases for LC-MS or LC-MS/MS bioanalysis
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Use of high-pH (basic/alkaline) mobile phases for LC-MS or LC-MS/MS bioanalysis

机译：使用LC-MS或LC-MS / MS生物分析的高pH（碱性/碱性）移动阶段

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摘要

High-pH or basic/alkaline mobile phases are not commonly used in LC-MS or LC-MS/MS bioanalysis because of the deeply rooted concern with column instability and reduced detection sensitivity for basic compounds in high-pH mobile phases owing to charge neutralization. With the advancement of LC column technology and the wide recognition of the "wrong-way-round" phenomena, high-pH mobile phases are more and more used in LC-MS or LC-MS/MS bioanalysis to improve chromatographic peak shape, retention, selectivity, resolution, and detection sensitivity, not only for basic compounds, but also for many other compounds. In this article, the benefits, practical considerations, application examples and cautions for using high-pH mobile phases in LC-MS or LC-MS/MS bioanalysis are reviewed, with a focus on quantification. Furthermore, the future trends in this field are also envisaged. A total of 84 references are cited in this review.
机译：由于充电中和，高pH或LC-MS / MS生物分析不常用于LC-MS或LC-MS / MS / MS生物分析中的高pH或碱性流动相。由于电荷中和，对高pH移动相的碱性化合物的检测灵敏度降低 。 随着LC柱技术的进步和广泛识别“错误方式”现象，高pH移动相越来越多地用于LC-MS或LC-MS / MS生物分析，以改善色谱峰形状，保留 ，选择性，分辨率和检测灵敏度，不仅适用于碱性化合物，还用于许多其他化合物。 在本文中，综述了使用LC-MS或LC-MS / MS生物分析中使用高pH移动相的益处，实践考虑，应用实例和注意事项，重点是定量。 此外，还设想了该领域的未来趋势。 本次审查中引用了84个参考文献。

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