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首页> 外文期刊>Cytokine >Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes.
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Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes.

机译：LIF拮抗剂在LIF和OSM诱导的初生关节软骨细胞表达MMP-1，MMP-3和TIMP-1中的作用。

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摘要

Cartilage degradation is mediated by matrix metalloproteinases (MMPs) and their inhibitors, tissue metalloproteinases (TIMPs), which are transcriptionally regulated by a variety of growth factors and cytokines. The levels of various MMPs as well as TIMPs have been shown to increase in response to certain cytokines. These include leukaemia inhibitory factor (LIF) and Oncostatin M (OSM), both of which have been detected in the synovial fluids of patients with rheumatoid arthritis (RA). However, the role of LIF and OSM in the regulation of various MMPs and TIMPs is still incompletely understood. The aims of this study were to examine the effects of LIF and OSM on MMP-1, MMP-3, and TIMP-1 production. In addition, the capacity of the LIF antagonist, MH35-BD, to block LIF and OSM induced MMP expression was examined. Primary chondrocytes, isolated from porcine metacarpophalangeal cartilage, were cultured in the presence and absence of LIF and OSM, with and without a predetermined concentration of the LIF antagonist. We analysed the levels of MMP-1, MMP-3 and TIMP-1 expression using qRT-PCR, Northern blot, and ELISA assays. The results indicate that LIF and OSM increase the expression of MMP-1, MMP-3, and TIMP-1 several fold. Furthermore their expression is reduced to basal levels in the presence of the LIF antagonist MH35-BD.
机译：软骨降解是由基质金属蛋白酶（MMP）及其抑制剂组织金属蛋白酶（TIMP）介导的，它们受多种生长因子和细胞因子的转录调控。各种MMP以及TIMP的水平已显示出对某些细胞因子的响应而增加。这些包括白血病抑制因子（LIF）和制瘤素M（OSM），在类风湿关节炎（RA）患者的滑液中都检测到了这两种物质。但是，LIF和OSM在调节各种MMP和TIMP中的作用仍不完全清楚。这项研究的目的是检查LIF和OSM对MMP-1，MMP-3和TIMP-1产生的影响。另外，检查了LIF拮抗剂MH35-BD阻断LIF和OSM诱导的MMP表达的能力。在有和没有预定浓度的LIF拮抗剂的情况下，在有和没有LIF和OSM的情况下培养从猪掌指软骨分离的原代软骨细胞。我们使用qRT-PCR，Northern印迹和ELISA分析了MMP-1，MMP-3和TIMP-1的表达水平。结果表明，LIF和OSM使MMP-1，MMP-3和TIMP-1的表达增加数倍。此外，在LIF拮抗剂MH35-BD存在下，它们的表达降低至基础水平。

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