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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Development of a simple method for simultaneous determination of tazarotene and betamethasone dipropionate and their metabolites using LC–MS method and its application to dermatopharmacokinetic study
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Development of a simple method for simultaneous determination of tazarotene and betamethasone dipropionate and their metabolites using LC–MS method and its application to dermatopharmacokinetic study

机译：用LC-MS法同时测定塔拉替烯和倍丙酮二丙酸酯二双丙酸酯及其代谢物的简单方法及其在Dermatopharmococetic研究中的应用

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Abstract In our study, a method for the determination for tazarotene and betamethasone dipropionate in human tissue‐engineered skin was established. Tazarotene gel, betamethasone dipropionate cream or a combination cream was administered to the skin. Then the skin was taken off at 0.25, 0.75, 1.75, 3, 5, 8, 12, 24, 36, 48?h time points after the residual drug was removed. The concentrations of tazarotene, betamethasone dipropionate and their major metabolites in skin were determined by LC–MS. Tazarotene and tazarotenic acid were detected in the concentration range of 2–200?μg/mL with an LLOQ of 2?μg/mL. Betamethasone dipropionate was detected in the concentration range 0.5–300?μg/mL with an LLOQ of 0.5?μg/mL, and betamethasone was detected at 2–200?μg/mL with an LLOQ of 2?μg/mL. The intra‐ and inter‐day precisions of the four analytes in the skin homogenate were all 15% (RSD, %). The results showed that tazarotene could be metabolized to tazarotenic acid and betamethasone dipropionate could be metabolized to betamethasone in tissue‐engineered skin. The results also revealed that this method was suitable for the simultaneous determination of tazarotene, betamethasone dipropionate and their metabolites in tissue‐engineered skin.
机译：摘要在我们的研究中，建立了一种测定塔拉替烯和雌滴鼻喉滴水在人体组织工程皮肤中的方法。将塔拉替烯凝胶，β徒甲基双丙酸酯霜或组合乳膏施用于皮肤。然后在除去残留药物后，在0.25,0.75,1.75,3,5,8,12,24,36,48Ω·H时间点下取出皮肤。通过LC-MS测定皮肤中塔拉替烯，β徒塞米松双丙酸酯和它们的主要代谢物的浓度。在2-200Ωμg/ ml的浓度范围内检测到塔扎拉塔罗替烯和达拉塔扎沙氨酸，LLOQ为2·μg/ mL。在浓度范围内检测到倍络滴滴量二倍体，在0.5-300Ω·μg/ ml，LLOQ，0.5Ω×μg/ ml，并在2-200Ω×μg/ ml下检测β甲基塞酮，LLOQ为2·μg/ ml。皮肤匀浆中的四种分析物的血液和日间精确均为均<15％（RSD，％）。结果表明，塔拉替烯可以代谢到唑塔塔基甲酸，并且可以将倍甲岛二丙酸二倍体代谢至组织工程皮肤中的雌滴体。结果还表明，该方法适用于同时测定塔拉替烯，脂肪醇二丙酸酯及其代谢物在组织工程皮肤中。

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