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首页> 外文期刊>Cytokine >Induction of type 1 interferon receptor by zinc in U937 cells.
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Induction of type 1 interferon receptor by zinc in U937 cells.

机译：锌在U937细胞中诱导1型干扰素受体。

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摘要

This study aims to determine whether zinc enhances interferon (IFN)-alpha activity in U937 cells. Type 1 IFN2 receptor (IFNAR2) protein in U937 cells was measured by flow cytometry. After 24h of exposure to zinc chloride or polaprezinc (a chelate of zinc and L-carnosine) at concentrations ranging from 50 to 200 microM, histograms showing anti-IFNAR2 antibody-positive cells shifted to a higher FITC intensity. Zinc chloride and polaprezinc increased IFNAR2 mRNA levels approximately 30% and 40%, respectively, compared to the control. L-carnosine alone did not alter IFNAR2 mRNA or protein levels. Cellular levels of 2'-5' oligoadenylate synthetases (OAS) were markedly increased by IFN-alpha, and the increase was significantly accelerated by polaprezinc. However, polaprezinc alone did not increase 2'-5'OAS levels. The finding suggests that zinc, especially polaprezinc, enhances the expression of INFAR2 in U937 cells, thereby inducing production of the anti-viral protein 2'-5'OAS.
机译：这项研究旨在确定锌是否能增强U937细胞中的干扰素（IFN）-α活性。通过流式细胞术测量U937细胞中的1型IFN2受体（IFNAR2）蛋白。暴露于浓度为50至200 microM的氯化锌或Polaprezinc（锌和L-肌肽的螯合物）24小时后，直方图显示抗IFNAR2抗体阳性的细胞转移至更高的FITC强度。与对照组相比，氯化锌和polaprezinc分别提高了IFNAR2 mRNA水平约30％和40％。单独的L-肌肽不会改变IFNAR2的mRNA或蛋白质水平。 IFN-α显着提高了2'-5'寡腺苷酸合成酶（OAS）的细胞水平，而波来普嗪显着促进了该水平的升高。但是，单独使用泊洛普锌不能增加2'-5'OAS的水平。该发现表明锌，特别是波来普锌，增强了U937细胞中INFAR2的表达，从而诱导了抗病毒蛋白2'-5'OAS的产生。

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