...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Comparison of 6-mercaptopurine with 6-thioguanine for the analysis of thiopurine S-methyltransferase activity in human erythrocyte by LC-MS/MS
【24h】

Comparison of 6-mercaptopurine with 6-thioguanine for the analysis of thiopurine S-methyltransferase activity in human erythrocyte by LC-MS/MS

机译：用LC-MS / MS对6-硫屈酮与6-硫瓜素硫氨酸S-甲基转移酶活性分析的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Thiopurines (TPDs) are first-line drugs in treating neuromyelitis optica spectrum disorders (NMOSD). Evaluation of thiopurine S-methyltransferase activity (TPMT), a major determinant of TPD toxicity, before TPD treatment using 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) as substrate was suggested. However, the equivalent of the two substrates in TPMT activity evaluation was unknown, and an alternative substrate was required in TPMT activity evaluation in patients who were already taking 6-MP or 6-TG. Before evaluating the agreement of 6-MP and 6-TG in TPMT activity measurement in patients with NMOSD, the affinity of the two substrates for the active center of TPMT should be established. A computer-based simulation indicated that 6-MP and 6-TG had similar affinities for the two active sites of TPMT. According to the guidelines, an LC-MS/MS method was developed and validated to evaluate the TPMT activity in human erythrocyte hemolysate using 6-MP or 6-TG as substrates via 1h incubation at 37 degrees C. The method was applied in 81 patients with NMOSD. Evaluated by Bland-Altman plot, 6-methylmercaptopurine and 6-methylthioguanine represented TPMT activities were in agreement with each other. Further studies are warranted to confirm the results.
机译：硫嘌呤（TPDS）是治疗神经髓炎OPTICA谱紊乱（NMOSD）的一线药物。拟提出了用6-巯基嘌呤（6-MP）和6-硫屈（6-TG）作为底物的TPD治疗前TPD毒性的主要决定蛋白S-甲基转移酶活性（TPMT）的评价。然而，TPMT活性评估中的两种底物的等同物未知，并且在已经服用6-MP或6-TG的患者中，在TPMT活性评估中需要替代底物。在NMOSD患者中评估6-MP和6-TG的6-MP和6-TG的协议之前，应建立两个基材的TPMT活跃中心的亲和力。基于计算机的仿真表明，6-MP和6-TG对于TPMT的两个有源网站具有类似的亲和力。根据指导原则，开发并验证了LC-MS / MS方法，以使用6-MP或6-Tg在37℃温育中使用6-MP或6-Tg作为底物评估人红细胞溶血物的TPMT活性。该方法应用于81例患者用nmosd。由Bland-Altman Plot评估，6-甲基汞型嘌呤和6-甲基硫叶绿素代表TPMT活动彼此一致。需要进一步研究以确认结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号