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首页> 外文期刊>Biomedical Chromatography: An International Journal Devoted to Research in Chromatographic Methodologies and Their Applications in the Biosciences >Fast and parallel determination of PCB 77 and PCB 180 in plasma using ultra performance liquid chromatography with diode array detection: A pharmacokinetic study in Swiss albino mouse
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Fast and parallel determination of PCB 77 and PCB 180 in plasma using ultra performance liquid chromatography with diode array detection: A pharmacokinetic study in Swiss albino mouse

机译：使用超级性能液相色谱法使用超级性能液相色谱法测定PCB 77和PCB 180的快速和平行测定二极管阵列检测：瑞士白化鼠的药代动力学研究

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摘要

A simple, sensitive and reproducible ultra-performance liquid chromatography (UPLC) method has been developed and validated for simultaneous estimation of polychlorinated biphenyl (PCB) 77 and PCB 180 in mouse plasma. The sample preparation was performed by simple liquid-liquid extraction technique. The analytes were chromatographed on a Waters Acquity H class UPLC system using isocratic mobile phase conditions at a flow rate of 0.3 mL/min and Acquity UPLC BEH shield RP18 column maintained at 35 degrees C. Quantification was performed on a photodiode array detector set at 215nm and PCB 101 was used as internal standard (IS). PCB 77, PCB 180, and IS retention times were 2.6, 4.7 and 2.8min, respectively, and the total run time was 6min. The method was validated for specificity, selectivity, recovery, linearity, accuracy, precision and sample stability. The calibration curve was linear over the concentration range 10-3000 ng/mL for PCB 77 and PCB 180. Intra- and inter-day precisions for PCBs 77 and 180 were found to be good with CV <4.64%, and the accuracy ranged from 98.90 to 102.33% in mouse plasma. The validated UPLC method was successfully applied to the pharmacokinetic study of PCBs 77 and 180 in mouse plasma.
机译：已经开发了一种简单，灵敏和可重复的超高效液相色谱（UPLC）方法，并验证用于在小鼠等离子体中同时估计多氯联苯（PCB）77和PCB 180。通过简单的液 - 液萃取技术进行样品制剂。通过以0.3ml / min的流速的异形流动相条件，在水分流速下进行分析物在水中进行色谱分离，并且在35℃的光电二极管阵列检测器上进行量化，在35摄氏度下保持的诸如UPLC BH屏蔽RP18柱的流速。并且PCB 101用作内标（是）。 PCB 77，PCB 180和保留时间分别为2.6,4.7和2.8min，总运行时间为6分钟。该方法是针对特异性，选择性，恢复，线性度，准确度，精度和样本稳定性验证的。校准曲线对于PCB 77和PCB 180的浓度范围为10-3000ng / ml，发现PCB 77和180的内部和日间诊断良好，CV <4.64％，精度范围小鼠等离子体中的98.90至102.33％。经过验证的UPLC方法被成功应用于小鼠等离子体中PCBS 77和180的药代动力学研究。

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