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首页> 外文期刊>Cytokine >The modulative effect of Cyperi Rhizoma on Th1/Th2 lineage development.
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The modulative effect of Cyperi Rhizoma on Th1/Th2 lineage development.

机译：香附对Th1 / Th2谱系发育的调节作用。

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To evaluate the direct effects of Cyperi Rhizoma (CR), a plant water extract, on Th1/Th2 lineage development in vitro, this study was conducted. Sorted CD4(+) T cells obtained from the splenocytes of BALB/c mice were activated with anti-CD3/anti-CD28 and then cultured in medium that contained CR medium under Th1 inducing or Th2 inducing conditions. Subsequently, IFN-gamma or IL-4 secreting cells were quantitated using flow cytometry analysis. In addition, IFN-gamma and IL-4 protein secretions were detected by ELISA analysis, after which, IFN-gamma and T-bet transcripts, key players in the Th1 immune function, and also, IL-4 and GATA-3, which are primary components in the Th2 immune mechanism, were quantitated by real-time RT-PCR. CR had no mitogenic effects on un-stimulated CD4(+) T cells, however, it increased the CD4(+) T cell population. Th1/Th2 polarization experiments revealed that CR enhanced IFN-gamma secretion in Th1 cells, but reduced the IL-4 in Th2 cells, and this occurred in a dose-dependent manner and showed significances. In addition, under Th1/Th2 skewed conditions, the transcription levels of IFN-gamma and T-bet were considerably increased, while the expressions of IL-4 and GATA-3 were relatively decreased with CR treatment. These findings suggest that CR enhances Th1 lineage development by increasing Th1 specific cytokine expression and secretion and reduces Th2 lineage development by repressing Th2 specific cytokine productions. Therefore, CR extract may be useful for preventing the onset of allergies or improving allergic symptoms.
机译：为了评估植物水提取物香附子对体外Th1 / Th2谱系发育的直接影响，进行了这项研究。从BALB / c小鼠脾细胞获得的分选的CD4（+）T细胞用抗CD3 /抗CD28激活，然后在Th1诱导或Th2诱导条件下在含有CR培养基的培养基中培养。随后，使用流式细胞仪分析对IFN-γ或IL-4分泌细胞进行定量。此外，通过ELISA分析检测到IFN-γ和IL-4蛋白的分泌，然后检测到IFN-γ和T-bet转录物，Th1免疫功能的关键因素，以及IL-4和GATA-3。是Th2免疫机制的主要成分，通过实时RT-PCR进行定量。 CR对未刺激的CD4（+）T细胞没有促有丝分裂作用，但是，它增加了CD4（+）T细胞的数量。 Th1 / Th2极化实验表明，CR增强了Th1细胞中IFN-γ的分泌，但减少了Th2细胞中的IL-4，这以剂量依赖的方式发生并具有重要意义。此外，在Th1 / Th2倾斜的条件下，CR处理使IFN-γ和T-bet的转录水平显着增加，而IL-4和GATA-3的表达则相对降低。这些发现表明CR通过增加Th1特异性细胞因子的表达和分泌而增强了Th1谱系的发育，并通过抑制Th2特异性细胞因子的产生而降低了Th2谱系的发育。因此，CR提取物可用于预防过敏发作或改善过敏症状。

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