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首页> 外文期刊>Cytokine >Modulation of Th1/Th2 cytokines and inflammatory mediators by hydroxychavicol in adjuvant induced arthritic tissues.
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Modulation of Th1/Th2 cytokines and inflammatory mediators by hydroxychavicol in adjuvant induced arthritic tissues.

机译：羟基chavicol在佐剂诱导的关节炎组织中对Th1 / Th2细胞因子和炎性介质的调节。

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摘要

The present study was undertaken to investigate the anti-arthritic activity of hydroxychavicol (HC) a major phenolic compound isolated from the aqueous extract leaves of plant Piper betle (Piperaceae). The compound showed significant lowering of pro-inflammatory (Th1) cytokine levels in arthritic paw tissue homogenate supernatant viz. IL-2, IFN-gamma, and TNF-alpha with maximum inhibition at higher dose levels of 2 and 4 mg/kg p.o. and enhanced the production of anti-inflammatory (Th2) cytokines IL-4 and IL-5 estimated by cytometric bead array immunoassay. Cytometric bead array uses the sensitivity of amplified fluorescence detection by flowcytometer to measure soluble analytes in a particle based immune assay. This assay can accurately quantitate five cytokines in a 50-microl sample volume. The T-helper (Th1) deviated cells produce detectable level of tumor necrosis factor (TNF-alpha), interleukin-2 (IL-2), and interferon-gamma (IFN-gamma), while the Th2 deviated cells produce significant amount of interleukin-4 (IL-4) and interleukin-5 (IL-5). HC at graded doses also significantly decreased the expression of IL-1beta, PGE(2), LTB(4), and nitric oxide levels showing significant inhibition of these parameters. Elevated levels of CD4(+) T cell specific interferon-gamma (IFN-gamma) in splenocytes of arthritic animals was also inhibited in treated animals. The oral LD(0) in both mice and rats was more than 1000 mg/kg.
机译：进行本研究是为了研究从植物胡椒（Piperaceae）的水提取物叶中分离出的主要酚类化合物羟基chavicol（HC）的抗关节炎活性。该化合物显示出关节炎足组织匀浆上清液中促炎（Th1）细胞因子水平的显着降低。 IL-2，IFN-γ和TNF-α在2和4 mg / kg p.o的较高剂量水平下具有最大抑制作用。并通过细胞计数磁珠阵列免疫测定法评估了抗炎（Th2）细胞因子IL-4和IL-5的产生。细胞计数珠阵列利用流式细胞仪检测扩增荧光的灵敏度，以基于颗粒的免疫测定法测量可溶性分析物。此测定法可以准确定量50微升样品体积中的五种细胞因子。 T辅助（Th1）偏离的细胞产生可检测水平的肿瘤坏死因子（TNF-alpha），白介素2（IL-2）和干扰素-γ（IFN-γ），而Th2偏离的细胞产生大量的肿瘤坏死因子（TNF-α）。白介素4（IL-4）和白介素5（IL-5）。分级剂量的HC还可以显着降低IL-1beta，PGE（2），LTB（4）和一氧化氮的表达，从而显着抑制这些参数。在治疗的动物中，关节炎动物脾细胞中CD4（+）T细胞特异性干扰素-γ（IFN-γ）的水平升高也受到抑制。小鼠和大鼠的口服LD（0）均超过1000 mg / kg。

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