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首页> 外文期刊>Cytometry, Part A: the journal of the International Society for Analytical Cytology >In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes
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In vivo multispectral, multiparameter, photoacoustic lymph flow cytometry with natural cell focusing, label-free detection and multicolor nanoparticle probes

机译：体内多光谱，多参数，光声淋巴流式细胞术，具有天然细胞聚焦，无标记检测和多色纳米颗粒探针

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Compared with blood tests, cell assessment in lymphatics is not well-established. The goal of this work was to develop in vivo lymph tests using the principles of flow cytometry. Cells in living animals were counted by laser (420-2,300 run) generation of photoacoustic (PA) signals in individual cells hydrodynamically focused by lymph valves into a single file flow, and using endogenous absorption as intrinsic cell-specific markers, or gold nanorods, nanoshells, and carbon nanotubes as multicolor probes. PA data were verified by high-speed transmission, photothermal, and fluorescent imaging. Counting of melanoma and immune-related cells in normal, apoptotic, and necrotic states in lymphatics in vivo was demonstrated to have the unprecedented sensitivity as one metastatic cell among millions of white blood cells. The time-resolved PA spectral identification of flowing cells was achieved using multicolor labels and laser pulses of different wavelengths and time delays. Multiparameter, noninvasive, portable flow cytometer can be used for preclinical Studies on animals with the potential of translation to humans for in vivo PA mapping of colorless lymph vessels and sentinel nodes with simultaneous single cell detection and metastasis assessment without labeling or use of contrast dyes and/or novel low-toxic multicolor probes with different absorption spectra. (C) 2008 International Society for Advancement of Cytotmetry.
机译：与血液检查相比，淋巴管细胞评估尚不完善。这项工作的目的是利用流式细胞仪原理开发体内淋巴试验。通过激光（420-2,300运转）对活细胞中的细胞进行计数，这些信号是通过淋巴阀以流体动力学方式聚焦到单个文件流中的单个细胞中产生的光声（PA）信号，并使用内源性吸收作为固有的细胞特异性标记物或金纳米棒纳米壳和碳纳米管作为多色探针。通过高速传输，光热和荧光成像验证了PA数据。体内淋巴瘤中正常，凋亡和坏死状态的黑色素瘤和免疫相关细胞计数被证明具有作为数百万个白细胞中的一种转移细胞前所未有的敏感性。使用多色标签和不同波长和时间延迟的激光脉冲可实现流动池的时间分辨PA光谱识别。多参数，非侵入性，便携式流式细胞仪可用于动物的临床前研究，该动物具有转化为人类的潜能，可用于无色淋巴管和前哨淋巴结的体内PA定位，同时进行单细胞检测和转移评估，而无需标记或使用造影剂/或具有不同吸收光谱的新型低毒多色探针。 （C）2008国际细胞计数技术进步学会。

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