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首页> 外文期刊>Breeding science >Reduced susceptibility to a tobacco bushy top virus Malawi isolate by loss of function in host eIF(iso)4E genes
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Reduced susceptibility to a tobacco bushy top virus Malawi isolate by loss of function in host eIF(iso)4E genes

机译：通过宿主EIF（ISO）4E基因的功能丧失，降低了对烟草浓密的顶部病毒马拉维分离物的易感性

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摘要

Tobacco bushy top disease (TBTD) is a viral disease of tobacco (Nicotiana tabacum L.) caused by mixed infection of Tobacco bushy top virus or Ethiopian tobacco bushy top virus and a helper virus. Despite its damage to tobacco, practical genetic resources for disease resistance have not been found. Here, we report that a mutation of tobacco eIF(iso)4E genes (eIF(iso)4E-S and eIF(iso)4E-T), which encode eukaryotic translation initiation factors, confers resistance (reduced susceptibility) to TBTD caused by a virus from Malawi (designated as tobacco bushy top virus Malawi isolate, TBTV-MW). RNAi lines in which eIF(iso)4E genes were silenced showed reduced susceptibility to TBTV-MW. We also tested chemically-induced single (eIF(iso)4E-S or eIF(iso)4E-T) and double (eIF(iso)4E-S and eIF(iso)4E-T) nonsense mutants for resistance to TBTV-MW. Suppression of eIF(iso)4E-S showed reduced susceptibility, and the resistance of the double mutant tended to be even stronger. eIF(iso)4E mutants also showed reduced susceptibility to TBTV-MW transmitted by aphids. To the best of our knowledge, the eIF(iso)4E-S mutant is the first genetic resource for TBTD resistance breeding in tobacco.
机译：烟草浓密的顶级疾病（TBTD）是由烟草浓密的烟草浓密病毒或埃塞俄比亚烟草浓密顶部病毒和辅助病毒的混合感染引起的烟草病毒疾病（Nicotiana Tabacum L.）。尽管其损坏了烟草，但尚未发现抗病性的实际遗传资源。在这里，我们报告说，烟草EIF（ISO）4E基因（EIF（ISO）4E-S和EIF（ISO）4E-T）的突变，它们编码真核转化起始因子，赋予TBTD的阻力（降低易感性）来自马拉维的病毒（指定为烟草浓密的顶部病毒马拉维分离物，TBTV-MW）。 RNAi线路，其中eif（ISO）4E基因沉默显示对TBTV-MW的易感性降低。我们还测试了化学诱导的单（EIF（ISO）4E-S或EIF（ISO）4E-T）和双（EIF（ISO）4E-S和EIF（ISO）4E-T）抗性突变体，用于抵抗TBTV- MW。抑制EIF（ISO）4E-S表示降低的敏感性，双突变体的电阻趋于更强。 EIF（ISO）4E突变体还表明对由蚜虫传输的TBTV-MW的易感性降低。据我们所知，EIF（ISO）4E-S突变体是烟草中TBTD抗性育种的第一个遗传资源。

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