...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Breeding science >Simple sequence repeat markers associated/linked with agronomic traits, as core primers, are eminently suitable for DNA fingerprinting in Upland cotton
【24h】

Simple sequence repeat markers associated/linked with agronomic traits, as core primers, are eminently suitable for DNA fingerprinting in Upland cotton

机译：与核心引物为核心引物相关/与农艺性状相关的简单序列重复标记物质适用于Upland棉中的DNA指纹识别

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Analyzing the genetic differences among crop germplasm resources scientifically and accurately is very important for the selection of core accessions, the identification of new cultivars, and the determination of seed purity. However, phenotypic selection per se is not sufficient to identify genetically distinct accessions. In this study, 26 out of 83 simple sequence repeat markers associated/linked with cotton important agronomic traits derived from our previous and other published research, corresponding to the 26 chromosomes of Upland cotton (Gossypium hirsutum L.), were selected as core primers for DNA fingerprinting construction. The 26 markers showed clear band patterns, good repeatability and high polymorphism. The average alleles, gene diversity index and polymorphism information content were 3.12, 0.4312 and 0.3830, respectively. Using TM-1, a genetic standard line for Upland cotton, as the control, DNA fingerprinting pattern and DNA barcodes were obtained based on the core primers. There was a significant positive correlation between genetic distance matrix determined using 26 core primers and that determined using more primers (335) derived from previous research, further suggesting that the core primers were eminently suitable for DNA fingerprinting in Upland cotton. This study provides a molecular basis for assessing identification, authenticity and seed purity of cotton cultivars.
机译：科学准确地分析作物种质资源之间的遗传差异对于选择核心摘要，鉴定新品种以及种子纯度的测定非常重要。然而，本身的表型选择是不足以识别基因上不同的脱析。在本研究中，与我们以前和其他公布的研究的棉花重要的农艺性质相关的83个简单的序列重复标记中的26个，选择了普通棉（Hostymium L.）的26个染色体作为核心引物DNA指纹识别结构。 26个标记显示出明显的带状图案，可重复性和高多态性。平均等位基因，基因多态性指数和多态性信息含量分别为3.12,0.4312和0.3830。使用TM-1，基于核心引物获得upland棉的遗传标准线，作为对照，DNA指纹图案和DNA条形码。使用26核引物测定的遗传距离基质之间存在显着的正相关性，并且使用来自先前研究的更多引物（335）确定的遗传距离基质（335）进一步表明核心引物在Upland棉中致核印刷件致命射门。本研究为评估棉花品种的鉴定，真实性和种子纯度提供了分子基础。

著录项

  • 来源
    《Breeding science》 |2018年第4期|共11页
  • 作者

    Li Chengqi; Chen Bihua; Xu Xinjuan; Li Dandan; Dong Jinyuan;

  • 作者单位

    Henan Inst Sci &

    Technol Henan Prov Cotton Res Inst Collaborat Innovat Ctr Modern Biol Breeding Xinxiang 453003 Henan Peoples R China;

    Henan Inst Sci &

    Technol Henan Prov Cotton Res Inst Collaborat Innovat Ctr Modern Biol Breeding Xinxiang 453003 Henan Peoples R China;

    Henan Inst Sci &

    Technol Henan Prov Cotton Res Inst Collaborat Innovat Ctr Modern Biol Breeding Xinxiang 453003 Henan Peoples R China;

    Henan Inst Sci &

    Technol Henan Prov Cotton Res Inst Collaborat Innovat Ctr Modern Biol Breeding Xinxiang 453003 Henan Peoples R China;

    Henan Inst Sci &

    Technol Henan Prov Cotton Res Inst Collaborat Innovat Ctr Modern Biol Breeding Xinxiang 453003 Henan Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 作物遗传育种与良种繁育;
  • 关键词

    simple sequence repeat markers (SSRs); DNA fingerprinting; DNA barcode; Upland cotton; core primer;

    机译：简单的序列重复标记（SSRS）;DNA指纹识别;DNA条形码;Upland棉;核心底漆;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号