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首页> 外文期刊>Breeding science >Allelic variation of low molecular weight glutenin subunits composition and the revealed genetic diversity in durum wheat (Triticum turgidum L. ssp. durum (Desf))
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Allelic variation of low molecular weight glutenin subunits composition and the revealed genetic diversity in durum wheat (Triticum turgidum L. ssp. durum (Desf))

机译:低分子量谷蛋白亚单元组成的等位基因变异及DURUM小麦遗传多样性(Triticum Turgidum L. SSP。硬粒(DESF))

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摘要

Low molecular weight glutenin subunits (LMW-GS) play an important role in determining the bread-making characteristics of dough in the end-use quality of wheat. In this study, A total of 149 worldwide-originated durum wheat were used to analyze the composition of LMW-GS using MALDI-TOF-MS. Based on the allelic variation of glutenin subunits, the genetic diversity was evaluated for the 149 durum wheat. Five types of alleles were identified at the Glu-A3 locus with Glu-A3e, Glu-A3a/c, Glu-A3f Glu-A3d and Glu-A3b accounting for 43.0%, 16.1%, 12.8%, 10.1% and 7.4% of the accessions, respectively. Five types of alleles were identified at the Glu-B3 locus: Glu-B3d (60.4%), Glu-B3b (6.0%), Glu-B3c (6.0%), Glu-B3h (2.7%) and Glu-B3f (0.7%). Two novel alleles encoding abnormal subunits 40500 Da and 41260 Da were identified at the Glu-A3 and Glu-B3 loci, respectively. Further studies are needed to match these novel alleles to previously discovered novel alleles. Moreover, the genetic diversity analysis indicated that great genetic variation existed in durum wheat among encoding loci of glutenin subunits, released periods of varieties and different geographical origins. The results provide more important information of potential germplasm for the improvement of durum wheat and common wheat.
机译:低分子量谷蛋白亚基(LMW-GS)在确定小麦的最终使用质量的面团的面团的面包特性方面发挥着重要作用。在这项研究中,共149个全球源于杜兰姆小麦使用MALDI-TOF-MS分析LMW-GS的组成。基于谷蛋白亚基的等位基因变异,对149个硬粒小麦评估了遗传多样性。在Glu-A3轨道上鉴定五种类型的等位基因,Glu-A3E,Glu-A3A / C,Glu-A3F Glu-A3D和Glu-A3B核算占43.0%,16.1%,12.8%,10.1%和7.4%分别进行加入。 Glu-B3基因座(60.4%),Glu-B3b(6.0%),Glu-B3C(6.0%),Glu-B3H(2.7%)和Glu-B3F(0.7 %)。在Glu-A3和Glu-B3基因座中鉴定了编码异常亚基40500Da和41260Da的两种新等位基因。需要进一步的研究以使这些新的等位基因与以前发现的新等位基因相匹配。此外,遗传多样性分析表明,在谷蛋白亚基的编码基因座,品种释放时期和不同地理起源中,杜兰姆小麦存在巨大的遗传变异。结果为改善杜兰姆小麦和普通小麦提供了更重要的信息。

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