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首页> 外文期刊>Cytokine >Il-8 and mcp-1 secretion is enhanced by the peptide-nucleic acid immunomodulator, product r, in u937 cells and primary human monocytes.
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Il-8 and mcp-1 secretion is enhanced by the peptide-nucleic acid immunomodulator, product r, in u937 cells and primary human monocytes.

机译：肽核酸免疫调节剂产物r在u937细胞和原代人单核细胞中增强了II-8和mcp-1的分泌。

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摘要

Product R (Reticulosetrade mark) is a peptide-nucleic acid immunomodulator recently shown to enhance the expression of mRNAs encoding pro-inflammatory cytokines. Interleukin 8 (IL-8) and macrophage chemoattractant protein-1 (MCP-1) are pro-inflammatory chemokines involved in immune cell mobilization and stimulation. To determine whether Product R acts by upregulating these chemokines, we assayed its effects on the expression of IL-8 and MCP-1 mRNAs and proteins by human monocytic U937 cells and by adherent peripheral blood mononuclear cells (PBMCs). U937 cells were cultured for 0-21 days in media containing 0-20% Product R or phosphate-buffered saline (PBS). Compared to control cultures, cells cultured in Product R expressed increased amounts of IL-8 and MCP-1 mRNAs, as measured by reverse transcriptase-polymerase chain reaction (RT-PCR). Product R also increased secretion of IL-8 and MCP-1, as measured by enzyme-linked immunosorbent assay (ELISA), and boosted secretion induced by bacterial lipopolysaccharide (LPS), in a time- and dose-dependent manner. In adherent PBMCs, Product R increased IL-8 and MCP-1 secretion, but reduced LPS-induced MCP-1 secretion. While mRNAs encoding the IL-8 receptor, CXCR2, and the MCP-1 receptor, CCR2, were increased in U937 cells cultured in 5-10% Product R, we observed no change in binding of receptor-specific antibodies. These findings suggest that Product R upregulates the expression of IL-8 and MCP-1, which may boost immune system activity in virally-infected patients. Copyright 2001 Academic Press.
机译：产品R（网状商标）是一种肽核酸免疫调节剂，最近被证明可以增强编码促炎性细胞因子的mRNA的表达。白细胞介素8（IL-8）和巨噬细胞趋化蛋白1（MCP-1）是参与免疫细胞动员和刺激的促炎性趋化因子。为了确定产物R是否通过上调这些趋化因子发挥作用，我们测定了其对人单核U937细胞和粘附的外周血单个核细胞（PBMC）对IL-8和MCP-1 mRNA和蛋白质表达的影响。将U937细胞在含有0-20％产品R或磷酸盐缓冲盐水（PBS）的培养基中培养0-21天。与对照培养相比，用逆转录酶-聚合酶链反应（RT-PCR）测量，产物R中培养的细胞表达的IL-8和MCP-1 mRNA量增加。通过酶联免疫吸附测定（ELISA）测定，产物R还以时间和剂量依赖性方式增加了IL-8和MCP-1的分泌，并增强了细菌脂多糖（LPS）诱导的分泌。在粘附的PBMC中，产物R增加IL-8和MCP-1分泌，但减少LPS诱导的MCP-1分泌。虽然在5-10％产品R中培养的U937细胞中，编码IL-8受体CXCR2和MCP-1受体CCR2的mRNA有所增加，但我们观察到受体特异性抗体的结合没有改变。这些发现表明，产物R上调IL-8和MCP-1的表达，这可能会提高病毒感染患者的免疫系统活性。版权所有2001，学术出版社。

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