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首页> 外文期刊>Cytokine >Effects of long-term estrogen treatment on ifn-gamma, il-2 and il-4 gene expression and protein synthesis in spleen and thymus of normal c57bl/6 mice.
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Effects of long-term estrogen treatment on ifn-gamma, il-2 and il-4 gene expression and protein synthesis in spleen and thymus of normal c57bl/6 mice.

机译：长期雌激素治疗对正常c57bl / 6小鼠脾脏和胸腺中ifn-γ，il-2和il-4基因表达及蛋白质合成的影响。

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Estrogens have been shown to markedly modulate the immune system. One mechanism by which estrogens could modulate the immune system is by regulating cytokines, an aspect not well-studied thus far. To address this issue, normal C57BL/6 orchiectomized mice were given estrogen and its effects on selected cytokines, interferon-gamma (IFN-gamma), interleukin 2 (IL-2) and IL-4 in lymphocytes from a developmental organ (thymus) and a mature lymphoid organ (spleen) examined. Estrogen significantly increased IFN-gamma and IL-2 mRNA in concanavalin-A (Con-A) activated thymocytes, splenic lymphocytes, and in enriched splenic T cells. Estrogen had no marked effect on IL-4 mRNA. While estrogen increased IFN-gamma mRNA in Con-A activated unseparated splenic lymphocytes and enriched splenic T cells, a numerical increase in IFN-gamma was noticed only in the supernatants of Con-A activated unseparated splenic lymphocytes, but not in enriched splenic T cells. This suggests that for optimal secretion of IFN-gamma in estrogen-treated mice, co-stimulatory signals from antigen presenting cells are needed. Gender differences in IFN-gamma and IL-2 mRNA were also evident. Con-A activated splenic lymphocytes from gonadal-intact, untreated female had a pattern of numerical increase in IFN-gamma mRNA, and IFN-gamma and IL-2 protein levels compared to their male counterparts. Taken together, our data suggests that estrogens regulate the expression of cytokines, which could account in part, for the gender differences in immune capabilities. Copyright 2001 Academic Press.
机译：已显示雌激素可显着调节免疫系统。雌激素调节免疫系统的一种机制是调节细胞因子，这一方面至今尚未得到充分研究。为了解决这个问题，正常的C57BL / 6睾丸切除小鼠被给予了雌激素，并且对发育器官（胸腺）淋巴细胞中的某些细胞因子，干扰素-γ（IFN-γ），白介素2（IL-2）和IL-4产生了影响。并检查成熟的淋巴器官（脾脏）。雌激素显着增加伴刀豆球蛋白A（Con-A）激活的胸腺细胞，脾淋巴细胞和富集的脾T细胞中的IFN-γ和IL-2 mRNA。雌激素对IL-4 mRNA没有明显影响。虽然雌激素增加了Con-A激活的未分离脾脏淋巴细胞和富集的脾T细胞中IFN-γmRNA的表达，但仅在Con-A激活的未分离的脾脏淋巴细胞的上清液中发现了IFN-γ的数值增加，而富集的脾脏T细胞中没有观察到。这表明，为了在雌激素治疗的小鼠中最佳地分泌IFN-γ，需要来自抗原呈递细胞的共刺激信号。 IFN-γ和IL-2 mRNA的性别差异也很明显。来自性腺完整，未经治疗的雌性患者的Con-A活化脾淋巴细胞与雄性相比具有IFN-γmRNA，IFN-γ和IL-2蛋白水平数值增加的模式。两者合计，我们的数据表明，雌激素调节细胞因子的表达，这可以部分解释免疫能力的性别差异。版权所有2001，学术出版社。

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