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首页> 外文期刊>Cytokine >Cigarette smoking depletes cells spontaneously secreting Th(1) cytokines in the human airway.
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Cigarette smoking depletes cells spontaneously secreting Th(1) cytokines in the human airway.

机译：抽烟会耗尽人类气道中自发分泌Th（1）细胞因子的细胞。

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Cigarette smoking may modify the immune balance in the airway since it alters the course of diseases in which immune system has an important role. This study examined whether cigarette smoking could affect the distribution of cells secreting Th(1) or Th(2) cytokines in the human airway. We utilized cytokine ELISPOT assay to detect and quantitate the frequencies of cells spontaneously secreting cytokines in bronchoalveolar lavage fluid (BALF). BALF was collected from six non-smokers and four heavy cigarette smokers without clinical airway symptoms. Cytokine ELISPOT assay was performed to quantitate cells secreting interleukin (IL-)2, IL-4 and interferon (IFN-)gamma with or without phorbor 12-myristate 13-acetate (PMA) stimulation. There were no cells spontaneously secreting IL-2 detected in all samples from smokers whereas most of non-smokers had detectable IL-2-secreting cells. The number of IFN-gamma-secreting cells was also extremely decreased in smokers. Mitogen-stimulated Th(1) cytokine-secreting cells were again significantly decreased in smokers' airways. The frequency of IL-2-secreting cells and CD4/CD8 ratio in BALF had a weak positive correlation. IL-4-secreting cells were not detected in any samples from both groups. These results show that cigarette smoking depletes Th(1) cytokine-secreting cells in the human airway. It may explain the susceptibility of smokers to certain airway disease conditions such as viral or mycobacterial infections and allergic diseases. Copyright 2001 Academic Press.
机译：吸烟可以改变呼吸道的免疫平衡，因为它改变了免疫系统起重要作用的疾病进程。这项研究检查了吸烟是否会影响人气道中分泌Th（1）或Th（2）细胞因子的细胞分布。我们利用细胞因子ELISPOT检测来检测和定量支气管肺泡灌洗液（BALF）中自发分泌细胞因子的细胞的频率。 BALF是从六名不吸烟者和四名重度吸烟者中收集的，没有临床呼吸道症状。进行细胞因子ELISPOT分析以定量在有或没有phorbor 12-肉豆蔻酸酯13-乙酸酯（PMA）刺激下分泌白介素（IL-）2，IL-4和干扰素（IFN-）γ的细胞。在所有吸烟者的样本中均未检测到自发分泌IL-2的细胞，而大多数非吸烟者均具有可检测到的IL-2分泌细胞。吸烟者中IFN-γ分泌细胞的数量也大大减少。在吸烟者的呼吸道中，丝裂原刺激的Th（1）细胞因子分泌细胞再次显着减少。 BALF中IL-2分泌细胞的频率和CD4 / CD8比率呈弱正相关。在两组的任何样品中均未检测到IL-4分泌细胞。这些结果表明，吸烟会耗尽人气道中Th（1）细胞因子的分泌细胞。它可以解释吸烟者对某些气道疾病状况的敏感性，例如病毒或分枝杆菌感染和过敏性疾病。版权所有2001，学术出版社。

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