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首页> 外文期刊>Cytokine >Plasticity of IL-2 and IL-2 receptor chains in rat lymphoid tissues in situ after stimulation with staphylococcal enterotoxin A.
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Plasticity of IL-2 and IL-2 receptor chains in rat lymphoid tissues in situ after stimulation with staphylococcal enterotoxin A.

机译：金黄色葡萄球菌肠毒素A刺激后大鼠淋巴组织中IL-2和IL-2受体链的可塑性。

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摘要

Although the effects of mitogens on the synthesis of interleukin-2 (IL-2) and IL-2 receptor (IL-2r) have been described, a detailed in situ analysis of the spatio-temporal changes of the expression of the IL-2 gene and the three IL-2r components in lymphoid tissues is still missing. Therefore, we analyzed the IL-2 and IL-2r expression after a staphylococcal enterotoxin A (SEA)-induced T cell activation on a cellular and anatomical basis in the Wistar rat. SEA caused a rapid induction of IL-2 mRNA in T cells of spleen, lymph node, and thymus, followed by the appearance of high systemic IL-2 serum levels (5 ng/ml), and a significant increase of CD25 on CD4(+) and CD8(+) lymphocytes. The histotopographic analysis of the IL-2r chains revealed a strong upregulation of IL-2r alpha (alpha) and IL-2r beta (beta) mRNAs in similar T cell specific compartments of spleen, lymph node, and thymus as seen for IL-2 mRNA. The abundant constitutive expression of IL-2r gamma (gamma) mRNA was unaffected by SEA. The parallel upregulation of IL-2, IL-2ralpha, and beta chains in conjunction with the continuous presence of the IL-2rgamma chain predominantly in T cell regions of immune organs suggests that the biological effects of IL-2 are essentially limited to T cells, at least after superantigen stimulation.
机译：尽管已经描述了促分裂原对白介素2（IL-2）和IL-2受体（IL-2r）合成的影响，但是对IL-2表达的时空变化进行了详细的原位分析基因和淋巴组织中的三个IL-2r成分仍然缺失。因此，我们在Wistar大鼠中基于细胞和解剖学分析了葡萄球菌肠毒素A（SEA）诱导的T细胞活化后的IL-2和IL-2r表达。 SEA引起脾脏，淋巴结和胸腺T细胞中IL-2 mRNA的快速诱导，随后出现高全身性IL-2血清水平（5 ng / ml），CD4上CD25显着增加（ +）和CD8（+）淋巴细胞。对IL-2r链的组织形态学分析显示，在脾脏，淋巴结和胸腺的类似T细胞特异性区室中，IL-2r alpha（alpha）和IL-2r beta（beta）mRNA的强烈上调。 mRNA。 SEA不会影响IL-2rγ（γ）mRNA的丰富组成型表达。 IL-2，IL-2ralpha和β链的平行上调以及主要在免疫器官T细胞区域中持续存在的IL-2rgamma链表明，IL-2的生物学作用基本上仅限于T细胞，至少在超抗原刺激后。

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