Brightly Fluorescent 2 '-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry

Guixens-Gallardo Pedro; Zawada Zbigniew; Matyasovsky Jan; Dziuba Dmytro; Pohl Radek; Kraus Tomas; Hocek Michal

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Brightly Fluorescent 2 '-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry

机译：明亮的荧光2' - 氧化氧基核苷三磷酸含甲基化BOBIPY荧光团的纤维素掺入DNA，成像和流式细胞术中

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Synthesis of cytosine, uracil, and 7-deazaadenine 2'-deoxyribonucleosides and triphosphates (dNTPs) bearing hexamethylated phenyl-bodipy fluorophore attached at position 5 of pyrimidines or at position 7 of 7-deazapurine was developed. All the title labeled nucleosides and dNTPs displayed bright green fluorescence with very high quantum yields. The modified dN(mBdp)TPs were good substrates to diverse DNA polymerases and were used for in vitro enzymatic synthesis of labeled DNA by primer extension or PCR. In combination with cationic cyclodextrin-peptide-based dNTP transporter, the dN(mBdp)TPs were successfully used for staining of genomic DNA in living cells for applications in confocal microscopy and in flow cytometry. The best performing cytosine nucleotide dN(mBdp)TPs was used to monitor mitosis in live cells.

机译：开发了胞嘧啶，尿嘧啶和7-二氮腺苷2'-脱氧核糖核苷的合成含有六甲基化苯基 - Bodipy荧光团，其含有六甲基化苯基 - Bodipy荧光团，或在7- ZeazapuRine的位置处的含有六甲基化苯基 - Bodipy荧光团。所有标题标记的核苷和DNTPS显示出具有非常高量子产率的亮绿色荧光。改性的DN（MBDP）TPS是良好的底物，用于各种DNA聚合酶，通过引物延伸或PCR用于标记DNA的体外酶合成。与阳离子环糊精肽的DNTP转运蛋白组合，DN（MBDP）TPS成功用于在共聚焦显微镜和流式细胞术中的活细胞中的基因组DNA染色。最佳性能的胞嘧啶核苷酸DN（MBDP）TPS用于监测活细胞中的含量。

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《Bioconjugate Chemistry》 |2018年第11期|共7页
作者
Guixens-Gallardo Pedro; Zawada Zbigniew; Matyasovsky Jan; Dziuba Dmytro; Pohl Radek; Kraus Tomas; Hocek Michal;
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Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

Czech Acad Sci Inst Organ Chem &

Biochem Flemingovo Nam 2 CZ-16610 Prague 6 Czech Republic;

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正文语种 eng
中图分类生物化学;
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1. Brightly Fluorescent 2 '-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry [J] . Guixens-Gallardo Pedro, Zawada Zbigniew, Matyasovsky Jan, Bioconjugate Chemistry . 2018,第11期

机译：明亮的荧光2' - 氧化氧基核苷三磷酸含甲基化BOBIPY荧光团的纤维素掺入DNA，成像和流式细胞术中
2. Synthesis of 2 '-deoxycytidine and its triphosphate bearing tryptophan-based imidazolinone fluorophore for environment sensitive fluorescent labelling of DNA [J] . Kuba Miroslav, Pohl Radek, Hocek Michal Tetrahedron . 2018,第46期

机译：合成2'-氧胞素胞苷及其三磷酸三磷酸含量的色氨酸咪唑啉酮荧光团，用于DNA的环境敏感荧光标记
3. Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. I. Chemical synthesis of various reporter group-labeled 2'-deoxyribonucleoside-5'-triphosphates [J] . Gerald Giller, Taurai Tasara, Bernhard Angerer, Nucleic Acids Research . 2003,第10期

机译：DNA聚合酶整合了报告分子标记的核苷酸。一，各种报告基团标记的2'-脱氧核糖核苷-5'-三磷酸酯的化学合成
4. Red and near-infrared fluorophores inspired by chlorophylls. Consideration of practical brightness in multicolor flow cytometry and biomedical sciences [C] . Masahiko Taniguchi, Gongfang Hu, Rui Liu, Conference on reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications . 2018

机译：受叶绿素启发的红色和近红外荧光团。多色流式细胞仪和生物医学中实际亮度的考虑
5. Bioimaging with Macromolecular Probes Incorporating Multiple BODIPY Fluorophores [O] . Ek Raj Thapaliya, Yang Zhang, Pravat Dhakal, -1

机译：结合多个BODIPY荧光团的高分子探针进行生物成像
6. Brightly Fluorescent 2′-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry [O] . Pedro Güixens-Gallardo, Zbigniew Zawada, Ján Matyašovský, 2018

机译：明亮的荧光2'-脱氧核糖核苷核苷三磷酸含有甲基化BOBIPY荧光团的纤维素掺入DNA，成像和流式细胞术中
7. Detailed Studies on the Application of Three Fluorescent Antibiotics for DNA Staining in Flow Cytometry [R] . Crissman, H. A., Stevenson, A. P., Orlicky, D. J., 1977

机译：三种荧光抗生素在流式细胞仪DNa染色中应用的详细研究

Brightly Fluorescent 2 '-Deoxyribonucleoside Triphosphates Bearing Methylated Bodipy Fluorophore for in Cellulo Incorporation to DNA, Imaging, and Flow Cytometry

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