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首页> 外文期刊>Cytokine >Comparative methods for multiplex analysis of cytokine protein expression in plasma of lipopolysaccharide-treated mice.
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Comparative methods for multiplex analysis of cytokine protein expression in plasma of lipopolysaccharide-treated mice.

机译：多重分析脂多糖治疗小鼠血浆中细胞因子蛋白表达的比较方法。

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摘要

Changes in circulating cytokines might serve as predictors of compound-evoked inflammatory responses. CD-1 mice were treated with lipopolysaccharide (LPS; 0.2 ml of 0.25 mg/ml, intraperitoneal) for subsequent expression measurement of plasma cytokine protein expression at 24-h post-treatment using multiple antibody Western blot, and at both 2-h and 24-h post-treatment using antibody array and suspension bead array. Antibody array provided a semi-qualitative assessment and suggested significantly increased expression of GCSF at 2-h post-treatment and GCSF, IL-6, IL-12, MCP-1, MCP-5, RANTES and sTNFR1 at 24-h post-treatment. Densitometric analysis of multiple antibody Western blots provided a semi-quantitative assessment and indicated significantly increased expression of IL-6, IL-12, IL-17, GCSF, eotaxin, and MCP-2 at 24-h post-treatment. The suspension bead array yielded statistically significant cytokine protein expression increases for IL-6, IL-10, IFNgamma and TNFalpha at both 2-h and 24-h post-treatments, while significant expression at 24-h post-treatment only was noted for IL-1beta, IL-5, IL-12 and GM-CSF. Suspension bead array provided the greatest range of detection, revealing subtle increased expression of GM-CSF, IL-1beta, IL-5, IL-10, TNFalpha and IFNgamma at 24-h post-treatment, not detected by antibody array or multiple antibody Western blot. Suspension bead array proved to be the best method for detection of LPS-evoked changes in plasma cytokine levels.
机译：循环细胞因子的变化可能是化合物诱发的炎症反应的预测因子。用脂多糖（CDS; 0.2 ml的0.25 mg / ml，腹膜内）处理CD-1小鼠，以便在治疗后24小时（使用多抗体Western印迹法）以及在2 h和2 h分别测量血浆细胞因子蛋白的表达。使用抗体阵列和悬浮珠阵列进行24小时后处理。抗体阵列可提供半定性评估，并建议在治疗后2小时显着增加GCSF的表达，并在治疗后24小时显着提高GCSF，IL-6，IL-12，MCP-1，MCP-5，RANTES和sTNFR1的表达。治疗。多种抗体Western印迹的光密度分析提供了半定量评估，并表明在治疗后24小时内IL-6，IL-12，IL-17，GCSF，嗜酸性粒细胞趋化因子和MCP-2的表达显着增加。悬浮珠阵列在治疗后2小时和24小时都产生IL-6，IL-10，IFNgamma和TNFalpha的统计学显着细胞因子蛋白表达增加，而仅在处理后24小时才发现显着表达IL-1beta，IL-5，IL-12和GM-CSF。悬浮珠阵列提供了最大的检测范围，显示在治疗后24小时内GM-CSF，IL-1beta，IL-5，IL-10，TNFalpha和IFNgamma的表达略有增加，而抗体阵列或多抗体未检测到蛋白质印迹。悬浮珠阵列被证明是检测LPS引起的血浆细胞因子水平变化的最佳方法。

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