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首页> 外文期刊>Biocontrol Science >Role of Phage Shock Protein in Recovery of Heat-injured Salmonella
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Role of Phage Shock Protein in Recovery of Heat-injured Salmonella

机译：噬菌体休克蛋白在热伤沙门氏菌中恢复的作用

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摘要

Sublethally heat-injured cells of Salmonella in food can recover under favorable conditions, leading to foodborne illness. To elucidate the molecular mechanism of recovery from heat injury, the global changes in gene transcription of Salmonella Typhimurium were investigated in previous study. In this study, the functions of genes involved in phage shock response (viz., phage shock protein (psp) genes), the transcription levels of which were found in previous study to be increased during recovery from heat injury, were investigated in recovering cells. The increase in pspABCDEFG transcription levels during the recovery process was confirmed by qRT-PCR. To understand the role of psp genes in heat injury recovery, a pspA deletion mutant (Delta pspA) and a pspA-overexpressing strain (S. Typhimurium pBAD30/pspA(+)) were constructed. Delta pspA showed slightly lower viable counts and membrane potential than those of the wild-type strain during recovery. On the other hand, there was no significant difference in the viable counts between S. Typhimurium pBAD30/pspA(+) and the control strains S. Typhimurium pBAD30/pspA (-) and S. Typhimurium pBAD30(+) during recovery. It would seem that a lack of PspA protein alone somewhat affects the recovery of S. Typhimurium from heat injury, but overexpression of PspA alone is not sufficient to overcome this effect.
机译：食物中的沙门氏菌的止氯热伤细胞可以在有利的条件下恢复，导致食源性疾病。为了阐明从热损伤中恢复的分子机制，在先前的研究中研究了沙门氏菌毛孢菌蕈脲基因转录的全局变化。在本研究中，噬菌体休克反应中涉及的基因的功能（噬菌体休克蛋白（PSP）基因），在恢复细胞中恢复前一项研究中发现的转录水平在从热损伤中恢复增加。通过QRT-PCR确认回收过程中PSPabcdefg转录水平的增加。为了了解PSP基因在热损伤中的作用，构建了PSPA缺失突变体（Delta PSPA）和PSPA过度抑制菌株（S.TyphimuriumPBAD30 / PSPA（+））。 Delta PSPA显示出比在恢复过程中的野生型应变的可行计数和膜电位略低。另一方面，在恢复期间，S. Typhimurium PBAD30 / PSPA（+）和对照菌毒蕈PBAD30 / PBAD30 / PSPA（ - ）和S.Typhimurium PBAD30（+）的可行计数没有显着差异。似乎缺乏PSPA蛋白单独影响来自热损伤的伤寒伤寒的蛋白质，但单独的PSPA的过度表达不足以克服这种效果。

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  • 来源
    《Biocontrol Science》 |2018年第1期|共9页
  • 作者

    Cui Xiaowen; Wen Hsu-Ming Sherman; Kinoshita Yoshimasa; Koishi Shota; Isowaki Chika; Ou Liushu; Masuda Yoshimitsu; Honjoh Ken-ichi; Miyamoto Takahisa;

  • 作者单位

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

    Kyushu Univ Dept Biosci &

    Biotechnol Grad Sch Fac Agr Higashi Ku 6-10-1 Hakozaki Fukuoka 8128581 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物保护;
  • 关键词

    Phage shock protein; Heat injury; Salmonella; Recovery; Membrane potential;

    机译：噬菌体休克蛋白;热损伤;沙门氏菌;恢复;膜势;

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