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Evaluation of a Novel Chromogenic Selective Medium for the Improved Detection of Campylobacter from Stool Samples

机译：新型色度选择性培养基评价粪便样品弯曲杆菌改善检测

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BD mCCDA Clear-HT (CCHT; Nippon Becton Dickinson Company, Ltd.), a novel chromogenic selective medium was evaluated for its superior capacity to isolate Campylobacter jejuni/ coli. When CCHT was assessed using 142 microbes including 42 Campylobacter jejuni/ coli strains, all Campylobacter strains were found to form purple-colored colonies on CCHT whereas all the other microbes failed to grow. CCTH was then compared with commercially available selective media using 100 stool samples including 40 Campylobacter positive samples. CCHT detected Campylobacter jejuni/ coli from 39 of 40 (97.5%) stool samples whereas it allowed competitive bacteria to grow as false positive colonies from 1 (1.0%) of 100 stool samples. The values of relative sensitivity (%) and specificity (%) for CCHT were 97.5 and 98.3 in this study. Our results demonstrated that CCHT had the highest detection ratio for Campylobacter jejuni/ coli and the highest inhibition ratio against competitive bacteria among all selective media compared.
机译：BD MCCDA Clear-HT（CCHT; Nippon Becton Dickinson Company，Ltd。），一种新型的色氨酸选择性培养基被评估其优异的能力，以孤立Campylobacter Jejuni / Coli。当使用包含42个弯曲杆菌/大肠杆菌菌株的142微生物评估CCHT时，发现所有弯曲杆菌菌株在CCHT上形成紫色菌落，而所有其他微生物未能生长。然后将CCTH与商业上可获得的选择性培养基进行比较，其中包含100个镇定样品，包括40个弯曲杆菌阳性样品。 CCHT检测到Campylobacter Jejuni / Coli从40个（97.5％）粪便样本中的39个（97.5％）粪便样本，而它允许竞争性细菌从1（1.0％）的100个粪便样本中生长为假阳性菌落。 CCHT的相对敏感性（％）和特异性（％）的值为97.5和98.3在该研究中。我们的结果表明，CCHT对弯曲杆菌/大肠杆菌的检测率最高，并且在所有选择性培养基中对抗竞争性细菌的最高抑制比。

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