...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biomedical Engineering Letters >Dielectrophoretic force measurement of red blood cells exposed to oxidative stress using optical tweezers and a microfluidic chip
【24h】

Dielectrophoretic force measurement of red blood cells exposed to oxidative stress using optical tweezers and a microfluidic chip

机译：使用光学镊子和微流体芯片暴露于氧化胁迫的红细胞介电泳力测量

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AbstractRed blood cell (RBC) dysfunction is often associated with a pathological intervention, and it has been proposed as a critical risk factor for certain lethal diseases. Examining the cell viability of RBCs under various physiological conditions is essential and of importance for precise diagnosis and drug discovery in the field of medicine and pharmacy. In this paper, we report a new analytical method that employs dielectrophoretic (DEP) force measurements in absolute units to assess the viability, and potentially the functionality of RBCs. We precisely quantify the frequency-dependent DEP forces of the RBCs by using a micro-electrode embedded chip combined with optical tweezers. DEP characteristics are known to be well-correlated with the viability of biological cells, and DEP forces are measured in both fresh and long-term stored RBCs to investigate the effect that the storage period has on the cell viability. Moreover, we investigate the DEP behavior of RBCs when exposed to oxidative stress and verify whether EDTA protects the RBCs from an oxidant. From the experiments, it is found that the fresh RBCs without oxidative stress display very high DEP forces over the entire frequency range, exhibiting two cutoff frequencies. However, both the RBCs stored for the long-term period and exposed to oxidative stress reveals that there exist no significant DEP forces over the frequency range. The results indicate that the DEP forces can serve as a useful parameter to verify whether the RBCs in certain blood are fresh and not exposed to oxidative stress. Therefore, it is believed that our system can be applied to a diagnostic system to monitor the cell viability of the RBCs or other types of cells.]]>
机译：<！[CDATA [<摘要ID =“ABS1”语言=“EN”OutputMedium =“全部”> <标题>抽象 ara>红细胞（RBC）功能障碍通常与病理干预相关联，已经提出了某些致命疾病的关键危险因素。在各种生理条件下检查RBCS的细胞活力对于医学和药房领域的精确诊断和药物发现是必不可少的。在本文中，我们报告了一种新的分析方法，采用绝对单位采用介电泳（DEP）力测量来评估活力，并且可能是RBC的功能。通过使用微电极嵌入式芯片与光学镊子组合，精确地量化了RBC的频率相关的DEP力。已知Dem特征与生物细胞的活力良好相关，并且在新鲜和长期储存的RBC中测量Dem力，以研究储存周期对细胞活力的影响。此外，我们在暴露于氧化应激时探测RBCS的DEP行为，并验证EDTA是否保护RBC免受氧化剂。从实验开始，发现没有氧化应力的新鲜RBC在整个频率范围内显示出非常高的DEP力，表现出两个截止频率。然而，储存用于长期时段并暴露于氧化应激的RBC都显示出在频率范围内没有显着的DEP力。结果表明，DEP力可以用作验证某些血液中的RBC是否是新鲜的并且不暴露于氧化应激的有用参数。因此，据信我们的系统可以应用于诊断系统，以监测RBC或其他类型的细胞的细胞活力。 ]]>

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号