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首页> 外文期刊>Cytokine >Candida albicans strain-dependent modulation of pro-inflammatory cytokine release by in vitro oral and vaginal mucosal models
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Candida albicans strain-dependent modulation of pro-inflammatory cytokine release by in vitro oral and vaginal mucosal models

机译：体外口腔和阴道黏膜模型对白色念珠菌菌株的促炎性细胞因子释放的依赖性调节

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Candida albicans is a commensal organism at several sites and is a versatile, opportunistic pathogen. The underlying factors of pathogen and host associated with commensalism and pathogenicity in C. albicans are complex and their importance is largely unknown. We aimed to study the responses of oral epithelial (OEM) and vaginal epithelial models (VEM) to infection by oral and vaginal C. albicans strains to obtain evidence of inter-strain differences in pathogenicity and of site-specificity. Following inoculation of models, proinflammatory cytokines IL-1α, IL-1β, IL-6, IL-8 and prostaglandin E2 (PGE2) release were monitored and histological staining undertaken. Striking differences in strain behaviour and epithelial responses were observed. IL-1α, IL-1β and IL-8 release were significantly increased from the OEM in response to denture stomatitis strain NCYC 1467. Increased IL-8 release also followed infection of the OEM with both vaginal strains. Overall the VEM was relatively unresponsive to infection with either oral or vaginal strains under these conditions. Adherence and hyphal development were observed for all strains on both models although extensive, uniform tissue penetration was seen only with stomatitis strain NCYC 1467 on the OEM. Candidal strains were assayed for phospholipase (PL) and secreted aspartyl proteinase (SAP) activities where phospholipase (PL) activity was highest for strain NCYC 1467 although highest SAP activity was observed for vaginal strain NCPF 8112 in this assay. This is the first study to concurrently investigate cytokine production from oral and epithelial models using candidal strains originating from these respective mucosal sites from healthy and disease states. These data demonstrate significant differences in inflammatory responses of host epithelia to individual C. albicans strains.
机译：白色念珠菌是几种地方的共生生物，是一种多功能的机会病原体。与白色念珠菌的共鸣和致病性相关的病原体和宿主的潜在因素很复杂，其重要性在很大程度上尚不清楚。我们旨在研究口服和阴道白色念珠菌菌株感染口腔上皮（OEM）和阴道上皮模型（VEM）的反应，以获得菌株间致病性和部位特异性差异的证据。接种模型后，监测促炎细胞因子IL-1α，IL-1β，IL-6，IL-8和前列腺素E2（PGE2）的释放并进行组织学染色。观察到应变行为和上皮反应的惊人差异。 OEM对假牙口腔炎菌株NCYC 1467的反应，IL-1α，IL-1β和IL-8的释放均显着增加。两种阴道菌株感染OEM后，IL-8的释放也有所增加。总体而言，在这些条件下，VEM对口腔或阴道菌株的感染相对无反应。在两个模型上都观察到了所有菌株的粘附和菌丝发育，尽管只有OEM上的口腔炎菌株NCYC 1467才看到广泛，均匀的组织渗透。分析了念珠菌菌株的磷脂酶（PL）和分泌的天冬氨酰蛋白酶（SAP）活性，其中菌株NCYC 1467的磷脂酶（PL）活性最高，尽管在此测定中阴道菌株NCPF 8112的SAP活性最高。这是第一个同时使用源自健康和疾病状态的这些各自粘膜部位的念珠菌菌株同时研究口腔和上皮模型中细胞因子产生的研究。这些数据证明了宿主上皮对单个白色念珠菌菌株的炎症反应存在显着差异。

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