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首页> 外文期刊>Cytokine >Adiponectin induces CCL20 expression synergistically with IL-6 and TNF-α in THP-1 macrophages
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Adiponectin induces CCL20 expression synergistically with IL-6 and TNF-α in THP-1 macrophages

机译：脂联素与THP-1巨噬细胞中IL-6和TNF-α协同诱导CCL20表达

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摘要

Adiponectin (Ad) is an adipokine secreted from adipocytes. It is reported that Ad has many biological activities. However, its influence on inflammation is controversial. In the present study, we examined the influence of Ad on production of CCL20 from THP-1 macrophages. THP-1 macrophages were prepared from THP-1 monocytes by PMA treatment. THP-1 macrophages were cultured for 24. h with Ad, IL-6, or TNF-α alone or with combinations of Ad and cytokines. CCL20 mRNA expression was then determined by real-time PCR. Full-length Ad (fAd) slightly but significantly induced CCL20 mRNA expression, and interestingly, co-stimulation with fAd and IL-6 or with fAd and TNF-α synergistically increased the expression of CCL20 mRNA. We explored the mechanism behind the synergistic effect of fAd and these cytokines. fAd did not affect the expression of receptors for IL-6 and TNF, and IL-6 and TNF-α did not increase the expression of the receptor for Ad in THP-1 macrophages. The increased expression of CCL20 by fAd is much higher in THP-1 macrophages compared with THP-1 monocytes. Furthermore, MMP-12 production was increased by IL-6 and TNF-α in THP-1 macrophages but it was not detectable in THP-1 monocytes. Treatment of fAd with MMP-12 induced globular Ad (gAd), and the expression of CCL20 in THP-1 macrophages was increased more potently by gAd than by fAd. MMP inhibitor (UK370106) inhibited the expression of CCL20 induced by co-stimulation with fAd and IL-6 or TNF-α. In conclusion, gAd played an important role in CCL20 expression, and MMP-12 induced by IL-6 or TNF-α was involved in the synergistic effect of fAd and cytokines.
机译：脂联素（Ad）是从脂肪细胞分泌的脂肪因子。据报道，Ad具有许多生物活性。然而，其对炎症的影响是有争议的。在本研究中，我们研究了Ad对THP-1巨噬细胞产生CCL20的影响。通过TMA-1单核细胞通过PMA处理制备THP-1巨噬细胞。将THP-1巨噬细胞与单独的Ad，IL-6或TNF-α或与Ad和细胞因子的组合培养24小时。然后通过实时PCR确定CCL20 mRNA表达。全长Ad（fAd）轻微但显着诱导CCL20 mRNA表达，有趣的是，与fAd和IL-6或与fAd和TNF-α共同刺激协同增加CCL20 mRNA的表达。我们探索了fAd和这些细胞因子协同作用背后的机制。 fAd不会影响IL-6和TNF受体的表达，IL-6和TNF-α不会增加THP-1巨噬细胞中Ad受体的表达。与THP-1单核细胞相比，在THP-1巨噬细胞中，fAd增加的CCL20表达增加了。此外，IL-6和TNF-α在THP-1巨噬细胞中增加了MMP-12的产生，但在THP-1单核细胞中却无法检测到。用MMP-12诱导的球状Ad（gAd）处理fAd，通过gAd比通过fAd更有效地增加THP-1巨噬细胞中CCL20的表达。 MMP抑制剂（UK370106）抑制与fAd和IL-6或TNF-α共同刺激诱导的CCL20表达。总之，gAd在CCL20表达中起重要作用，IL-6或TNF-α诱导的MMP-12参与fAd和细胞因子的协同作用。

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