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首页> 外文期刊>Biocontrol Science and Technology >Spore toxin complex recovery from solid-state fermentation of some mosquitocidal Bacilli
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Spore toxin complex recovery from solid-state fermentation of some mosquitocidal Bacilli

机译：从一些蚊子杆菌的固态发酵孢子孢子复合恢复

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摘要

The efficiency of different methods for recovery of spore toxin complex (STC) from solid-state fermentation (SSF) extracts of Bacillus thuringiensis israelensis (Bti) and Lysinibacillus sphaericus 14N1(Ls14N1) was studied. Drying at 30–50°C, lyophilisation, flocculation by ferric chloride at 0.1–0.2% and encapsulation by entrapment in calcium alginate beads, agar, or polyacrylamide gel were efficient methods for recovery of STC of Ls14N1 and Bti from SSF extracts. Also, STC was precipitated with remained the mosquitocidal activity through adjusting the pH of the SSF liquid extract before centrifugation at pH 2–5 or after addition of acetone (200–400%), ethanol (400%) or acetic acid (2.5–4%) to SSF liquid extract of Ls14N1. On the other hand, STC of Bti showed a good recovery by centrifugation after adjusting SSF liquid extract at pH 4 followed by pH 3 and pH 5 or after the addition of ethanol (300%) or acetone (200–300%). This study contains simple and multiple methods for efficient recovery of STC of Bti and Ls14N1 grown under SSF. The choice of the best method depends on the availability of the necessary equipment as well as the cost of the treatment.
机译：研究了来自固态发酵（SSF）芽孢杆菌（SSF）以色列（BTI）和延生菌菌菌菌菌（BTI）和Lysinibacillus Sphaericus 14N1（LS14N1）的孢子毒素复合物（STC）的效率。在30-50℃下干燥，冷冻干燥，通过氯化铁的絮凝，并在藻酸钙珠粒，琼脂或聚丙烯酰胺凝胶中包封，是从SSF提取物中回收LS14N1和BTI的STC的有效方法。此外，通过调节在pH 2-5离心前的SSF液体提取物的pH或加入丙酮（200-400％），乙醇（400％）或乙酸（2.5-4）后（2.5-4 ％）LS14N1的SSF液体提取物。另一方面，BTI的STC通过在pH 4调节SSF液体提取物之后通过离心显示出良好的恢复，然后在pH 3和pH 5或加入乙醇（300％）或丙酮（200-300％）之后。本研究含有简单且多种方法，可在SSF下生长的BTI和LS14N1 STC的高效恢复。最佳方法的选择取决于必要设备的可用性以及治疗的成本。

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