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首页> 外文期刊>Biocontrol Science and Technology >Biocontrol potential of five Burkholderia and Pseudomonas strains against Colletotrichum truncatum infecting chilli pepper
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Biocontrol potential of five Burkholderia and Pseudomonas strains against Colletotrichum truncatum infecting chilli pepper

机译：五个伯克德列风和假单胞菌菌株的生物控制潜力对抗Collettrichum truncatum感染辣椒

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摘要

The effect of bacterial antagonism against the causal agent, Colletotrichum truncatum was assessed as a potential alternative in managing anthracnose in chilli pepper. Out of 104 contrasting bacterial colonies isolated from rhizosphere soil of a forest floor, five isolates caused the radial growth inhibition greater than 90% (Significant at p??0.05 level) of C.?truncatum in dual cultures. Based on 16S rRNA analysis, these antagonistic bacterial isolates were identified as Pseudomonas aeruginosa, Burkholderia arboris, Burkholderia gladioli and Burkholderia rinojensis. The selected promising antagonists showed nearly 100% inhibition of the spore germination of C.?truncatum in vitro. These antagonists produced antifungal compounds which are diffusible in nature. Microscopic studies of blackened fungal hyphae, which were subjected to antagonism showed many deformations such as thickening, swelling and malformation. In vivo study revealed that C.?truncatum inoculated chilli pepper seeds treated with the five antagonists significantly inhibited the incidence of seed colonisation (p??0.05) by the pathogen. At the post emergence stage, the survival percentages and vigour indices of all the antagonists’ treatments, except B.?rinojensis strain 1, were significantly higher compared to the untreated control. The efficacy of the selected antagonists in managing anthracnose fruit rot was 100% at the colour breaking stage of chilli pepper fruits. These bacterial antagonists had a negative effect on C.?truncatum spore attachment and subsequent colonisation on chilli pepper leaves except in the treatment of B.?arboris. The results of in-vitro and in-vivo studies, suggest that the screened antagonistic bacterial isolates are potential biocontrol agents and need to be further studied for the biochemical basis of their activity against C.?truncatum.
机译：细菌拮抗作用对因果剂的影响，Colletottrichum truncatum被评估为辣椒管理Anthracnose的潜在替代品。在104个对比的细菌菌落中，从森林地板的根际土壤中分离出来，在双重培养中，五个分离株导致径向生长抑制大于90％（在p≤≤0.05级）的C.?truncatum。基于16S RRNA分析，这些拮抗细菌分离株被鉴定为假单胞菌铜绿假单胞菌，Burkholderia Arboris，Burkholderia Gladioli和Burkholderia Rinojensis。所选择的拮抗剂在体外占C.Truncatum的孢子萌发近100％抑制。这些拮抗剂产生了性质上扩散的抗真菌化合物。对拮抗作用的黑色真菌菌丝的显微镜研究显示出许多变形，如增稠，溶胀和畸形。在体内研究表明，用五个拮抗剂治疗的C.?Truncatum接种辣椒种子显着抑制了病原体的种子定植的发生率（p≤≤0.05）。在出生后阶段，与未处理的对照相比，除了B.?Rinojensis菌株1外，所有拮抗剂治疗的生存百分比和活力指数显着高得多。在辣椒水果的颜色破碎阶段，所选拮抗剂在管理Anthracnose果实中的疗效是100％。这些细菌拮抗剂对C.Turruncatum孢子附着的负面影响以及随后在辣椒叶上的殖民化，除了治疗B.? arboris。体外和体内研究的结果表明，筛选的拮抗细菌分离物是潜在的生物控制剂，并且需要进一步研究其对C.?Truncatum活性的生化基础。

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