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Reconstituting National Institute for Biological Standards and Control (NIBSC) chemokines

机译:重组国家生物标准与控制研究所(NIBSC)趋化因子

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摘要

We observed significant discrepancies between immunoassay results when using different internally prepared reference preparations for interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) from the National Institute for Biological Standards and Control (NIBSC). To evaluate the reasons for this we prepared the chemokines using diluents that incorporated protein at different steps. This showed that even brief addition of water to these preparations, in the absence of additional protein, resulted in loss of immunoreactivity in assays. The data obtained emphasise the importance of adding protein at an early stage of preparation to avoid loss of material and potential loss of activity.
机译:当观察到不同的内部制备的白细胞介素8(IL-8)和美国国家生物标准与控制研究所(NIBSC)的单核细胞趋化蛋白1(MCP-1)的参考制剂时,免疫分析结果之间存在显着差异。为了评估其原因,我们使用在不同步骤掺入蛋白质的稀释剂制备了趋化因子。这表明,即使在没有其他蛋白质的情况下向这些制剂中短暂添加水,也会导致测定中免疫反应性的丧失。获得的数据强调了在制备的早期阶段添加蛋白质的重要​​性,以避免物质损失和潜在的活性损失。

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