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The promotional effect of IL-22 on mineralization activity of periodontal ligament cells

机译：IL-22对牙周膜细胞矿化活性的促进作用

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Objectives: Interleukin (IL)-22 acts on non-immune cells to induce anti-microbial responses, protection from tissue damage, and enhance cell regeneration. However, little is known about the involvement of IL-22 in periodontal biology. This study investigated the biological effects of IL-22 on periodontal ligament (PDL) cells as part of studies to assess the involvement of IL-22 in periodontal disease. Materials and methods: Gene expression levels of IL-22 and its receptors in PDL cells and gingival tissue samples were evaluated by real-time PCR. Proliferative responses and mineralized-matrix forming activities of PDL cells were examined in the presence and absence of IL-22. Results: In contrast to the expression of IL-22 receptors detected in PDL tissues and their cell lines, gingival tissues showed modest or no gene expressions of IL-22. The production of several cytokines including IL-11, IL-8 and CCL2 was upregulated by IL-22 treatment of PDL cells in a dose-dependent manner. IL-22 treatment had no effect on the proliferative response in PDL cells. Meanwhile, IL-22 precipitated mineralized nodule formation and induced gene expressions of RUNX2, MSX2 and osteocalcin in PDL cells, suggesting that IL-22 enhances the mineralized matrix-forming activities of PDL cells. Conclusion: IL-22 has the potential to promote mineralizing activity in PDL cells and to develop appropriate regenerative therapy.
机译：目标：白介​​素（IL）-22作用于非免疫细胞，以诱导抗微生物反应，防止组织损伤并增强细胞再生。然而，关于IL-22参与牙周生物学的知之甚少。这项研究调查了IL-22对牙周膜（PDL）细胞的生物学影响，作为评估IL-22参与牙周疾病的研究的一部分。材料与方法：通过实时荧光定量PCR检测PDL细胞和牙龈组织样品中IL-22及其受体的基因表达水平。在存在和不存在IL-22的情况下检查PDL细胞的增殖反应和矿化基质形成活性。结果：与在PDL组织及其细胞系中检测到的IL-22受体表达相反，牙龈组织显示IL-22的基因表达中等或没有。 IL-22处理PDL细胞以剂量依赖的方式上调了包括IL-11，IL-8和CCL2在内的几种细胞因子的产生。 IL-22处理对PDL细胞的增殖反应没有影响。同时，IL-22沉淀了PDL细胞中矿化结节的形成，并诱导RUNX2，MSX2和骨钙素的基因表达，提示IL-22增强了PDL细胞的矿化基质形成活性。结论：IL-22具有促进PDL细胞矿化活性和开发适当再生疗法的潜力。

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