...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cytokine >Resistance exercise modulates lipid plasma profile and cytokine content in the adipose tissue of tumour-bearing rats
【24h】

Resistance exercise modulates lipid plasma profile and cytokine content in the adipose tissue of tumour-bearing rats

机译：抵抗运动调节荷瘤大鼠脂肪组织中的脂质血浆分布和细胞因子含量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cancer cachexia is a multifactorial syndrome characterised by progressive weight loss, frequently accompanied by anorexia, sarcopenia, and chronic systemic inflammation. The white adipose tissue is markedly affected by cachexia and contributes to this syndrome throught the secretion of pro-inflammatory factors which reach the adjacent tissues and the circulation. A nonpharmacologic intervention that may attenuate cancer cachexia is chronic physical activity, but the effect of resistance training upon adipose tissue inflammation in cachexia has never been examined. For that purpose we designed a protocol in which animals were randomly assigned to a control group (CT, n=7), a Tumour bearing group (TB, n=7), a Resistance Trained group (RT, n=7) and a Resistance Trained tumour bearing group (RTTB, n=7). Trained rats climbed a vertical ladder with an extra load attached to the tail, representing 75-90% of total body mass, 3 times per week, for 8weeks. In the 6thweek of resistance training, tumour cells (3??107 Walker 256 carcinosarcoma) were inoculated in the tumour groups. Body, adipose tissue, muscle and tumour mass was determined, as well a blood biochemical parameters, and the hormone and cytokine profile assessed. The glycogen content of the liver and muscle was measured. IL-10, IL-6 and TNF-?? protein expression was evaluated in the mesenteric adipose tissue (MEAT) examined. Resistance training increased by 9% body weight gain in RTTB (final weight 310.8??9.8g), when compared with TB (final weight 288.3??4.9g). LDL-c levels were decreased in RTTB (0.28??0.9mmol/L) by 43% when compared with TB (0.57??0.1mmol/L). HDL-c levels were increased in RTTB (1.31??0.12mmol/L) by 15% in regard to CT (1.13??0.7mmol/L) and 22% as compared with TB (1.07??0.07mmol/L). RTTB testosterone levels (577??131ng/mL) were 55% higher when compared with CT (254??41.3ng/mL) and 63% higher when compared with TB (221??23.1ng/mL). Adiponectin levels were augmented in RT (23??g/mL) by 43% when compared with TB (11??g/mL). Protein expression of IL-6 was increased 38% in TB MEAT (5.95pg/??g), as compared with CT (3.64pg/??g) and 50% compared with RTTB (2.91pg/??g). Similar results with respect to TNF-?? TB (7.18pg/??g) were observed: 39% and 46%, higher protein expression in comparison with CT (4.63pg/??g) and RTTB (3.8pg/??g), respectively. IL-10 protein expression was found to be increased in TB (4.4pg/??g) and RTTB (3.2pg/??g) 50% and 47%, respectively, in comparison with CT (1.2pu/??g). The IL-10/TNF-?? ratio was higher in RTTB in relation to all others experimental groups. The results show a robust effect of resistance exercise training in preventing important symptoms of cancer cachexia, thus strongly suggesting it may appear as an alternative to endurance exercise as a non-pharmacological therapy in the management of this syndrome. ? 2012.
机译：癌症恶病质是一种多因素综合征，其特征在于进行性体重减轻，经常伴有厌食症，肌肉减少症和慢性全身性炎症。白色脂肪组织明显受恶病质的影响，并通过分泌促炎因子到达邻近组织和循环而促成该综合征。可以减轻癌症恶病质的一种非药物干预措施是慢性体育锻炼，但从未研究过抗药性训练对恶病质中脂肪组织炎症的影响。为此，我们设计了一种方案，其中将动物随机分为对照组（CT，n = 7），荷瘤组（TB，n = 7），抗性训练组（RT，n = 7）和对照组。抵抗力训练的荷瘤组（RTTB，n = 7）。受过训练的大鼠爬上垂直梯子，尾巴上有额外的负荷，占总体重的75-90％，每周3次，共8周。在抗性训练的第6周，在肿瘤组中接种肿瘤细胞（3×107 Walker 256癌肉瘤）。测定身体，脂肪组织，肌肉和肿瘤的质量，以及血液生化参数，并评估激素和细胞因子的分布。测量肝脏和肌肉的糖原含量。 IL-10，IL-6和TNF-α在检查的肠系膜脂肪组织（MEAT）中评估蛋白质表达。与TB（最终体重288.3〜4.9g）相比，RTTB（最终体重310.8〜9.8g）的阻力训练使体重增加了9％。与TB（0.57≤0.1mmol/ L）相比，RTTB（0.28≤0.9mmol/ L）的LDL-c水平降低了43％。 RTTB（1.31≤0.12mmol/ L）的HDL-c水平相对于CT（1.13≤0.7mmol/ L）增加了15％，与TB（1.07≤0.07mmol/ L）相比增加了22％。 RTTB的睾丸激素水平（577 ?? 131ng / mL）与CT（254 ?? 41.3ng / mL）相比增加了55％，与TB（221 ?? 23.1ng / mL）相比增加了63％。与结核菌相比，脂联素水平在RT（23？g / mL）中增加了43％（11？3g / mL）。与CT（3.64pg /Δg）相比，TB MEAT（5.95pg /Δg）的IL-6蛋白表达增加38％，与RTTB（2.91pg /Δg）相比，IL-6的蛋白表达增加50％。关于TNF-α的相似结果观察到TB（7.18pg /Δg）：39％和46％，分别比CT（4.63pg /Δg）和RTTB（3.8pg /Δg）更高的蛋白质表达。与CT（1.2pu /Δg）相比，发现TB（4.4pg /Δg）和RTTB（3.2pg /Δg）的IL-10蛋白表达分别增加了50％和47％。 。 IL-10 /TNF-α与所有其他实验组相比，RTTB中的比率更高。结果表明，抵抗运动训练在预防癌症恶病质的重要症状方面具有强大的作用，因此有力地表明，它可以作为耐力运动的替代品，作为治疗该综合征的一种非药物疗法。 ？ 2012。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号