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Human skeletal muscle releases leptin in vivo

机译：人体骨骼肌在体内释放瘦素

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Leptin is considered an adipokine, however, cultured myocytes have also been found to release leptin. Therefore, as proof-of-concept we investigated if human skeletal muscle synthesized leptin by measuring leptin in skeletal muscle biopsies. Following this, we quantified human skeletal muscle and adipose tissue leptin release in vivo. We recruited 16 healthy male human participants. Catheters were inserted into the femoral artery and vein draining skeletal muscle, as well as an epigastric vein draining the abdominal subcutaneous adipose tissue. By combining the veno-arterial differences in plasma leptin with measurements of blood flow, leptin release from both tissues was quantified. To induce changes in leptin, the participants were infused with either saline or adrenaline in normo-physiological concentrations. The presence of leptin in skeletal muscle was confirmed by western blotting. Leptin was released from leg skeletal muscle (50.6±12ngmin -1) and the pattern of release was different from subcutaneous adipose tissue. Moreover, during adrenaline infusion the leptin release from leg skeletal muscle was strongly suppressed (20.5±7.9ngmin -1, p0.017), whereas the release from fat was unaltered. During saline infusion the adipose tissue release averaged 0.8±0.3ngmin -1 100g tissue -1 whereas skeletal muscle release was 0.5±0.1ngmin -1 100g tissue -1. In young healthy humans, skeletal muscle contribution to whole body leptin production could be substantial given the greater mass of muscle compared to fat. An understanding of the role that leptin plays in skeletal muscle metabolism may prove important in light of several late-phase trials with recombinant leptin as an anti-obesity drug.
机译：瘦素被认为是脂肪因子，但是，还发现培养的心肌细胞会释放瘦素。因此，作为概念验证，我们研究了人体骨骼肌是否通过测量骨骼肌活检组织中的瘦素来合成瘦素。此后，我们量化了人体骨骼肌和脂肪组织瘦素的体内释放。我们招募了16位健康的男性人类参与者。将导管插入股动脉和引流骨骼肌，以及引流腹部皮下脂肪组织的上腹静脉。通过将血浆瘦素中的静脉-动脉差异与血流测量值结合起来，定量了从两个组织中释放出的瘦素。为了诱导瘦素发生变化，将正常生理浓度的生理盐水或肾上腺素注入受试者体内。通过蛋白质印迹证实了瘦蛋白在骨骼肌中的存在。瘦素从腿部骨骼肌中释放（50.6±12ngmin -1），且释放方式与皮下脂肪组织不同。此外，在肾上腺素输注期间，从腿骨骼肌中释放的瘦蛋白被强烈抑制（20.5±7.9ngmin -1，p <0.017），而从脂肪中的释放没有改变。在输注盐水期间，脂肪组织的平均释放为0.8±0.3ngmin -1 100g组织-1，而骨骼肌的释放为0.5±0.1ngmin -1 100g组织-1。在年轻健康的人类中，鉴于肌肉比脂肪更大，骨骼肌对全身瘦素产生的贡献可能很大。根据将重组瘦素作为抗肥胖药的几项后期试验，了解瘦素在骨骼肌代谢中的作用可能很重要。

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