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首页> 外文期刊>Cytokine >The role of interleukin 6 in interferon-gamma production in thermally injured mice.
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The role of interleukin 6 in interferon-gamma production in thermally injured mice.

机译：白细胞介素6在热损伤小鼠中干扰素-γ产生中的作用。

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Following traumatic injury, patients suffer from compromised immunity increasing their susceptibility to infection. Previous studies from this laboratory demonstrated that female BALB/c mice subjected to a 15% total body surface area (TBSA) scald injury exhibit a decrease in cell-mediated immunity 10 days post-burn. Studies described herein revealed that concanavalin A (Con A; 2 microg/ml)-stimulated splenocytes from sham treated animals produced 3557+/-853 pg/ml of IFN-gamma while splenocytes from burn injured animals released two-fold more cytokine (P<0.05). To determine whether leukocyte production of IFN-gamma was under the influence of macrophages, splenic macrophage supernatants generated from burned animals were incubated with splenic lymphocytes from sham and burn animals. The amount of IFN-gamma released by lymphocytes from sham animals increased when cultured with macrophages from burned mice (P<0.05). This suggests that the increase in IFN-gamma production by unfractionated splenocytes in burned mice relative to sham treated animals is macrophage-dependent. Macrophage supernatants from burned mice released twice as much IL-6 as supernatants from sham animals (P<0.05), and when IL-6 was blocked in vivo, the amount of IFN-gamma production in burned mice decreased to sham levels (P<0.05). Thus, IL-6 mediates IFN-gamma production following burn. Copyright 2000 Academic Press.
机译：遭受外伤后，患者的免疫力受损，容易感染。来自该实验室的先前研究表明，遭受15％全身表面积（TBSA）烫伤的雌性BALB / c小鼠在烧伤后10天表现出细胞介导的免疫力降低。本文所述研究表明，假手术处理动物的伴刀豆球蛋白A（Con A; 2 µg / ml）刺激的脾细胞产生3557 +/- 853 pg / mlIFN​​-γ，而烧伤动物的脾细胞释放的细胞因子增加了两倍（P <0.05）。为了确定IFN-γ的白细胞产生是否在巨噬细胞的影响下，将从烧伤动物产生的脾脏巨噬细胞上清液与假和烧伤动物的脾脏淋巴细胞一起孵育。与烧伤小鼠的巨噬细胞一起培养时，假动物的淋巴细胞释放的IFN-γ数量增加（P <0.05）。这表明相对于假手术处理的动物，烧伤小鼠中未分离的脾细胞产生的IFN-γ的增加是巨噬细胞依赖性的。烧伤小鼠的巨噬细胞上清液释放的IL-6是假动物上清液的两倍（P <0.05），并且当IL-6体内被阻断时，烧伤小鼠中的IFN-γ产生量降低到了假样品水平（P < 0.05）。因此，IL-6在烧伤后介导IFN-γ的产生。版权所有2000学术出版社。

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