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首页> 外文期刊>Cytokine >Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius)
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Comparison of virokine from camel pseudocowpoxvirus (PCPV) with Interleukin 10 of the Dromedary camel (Camelus dromedarius)

机译：骆驼伪牛痘病毒（PCPV）中的病毒因子与单峰骆驼（Camelus dromedarius）的白介素10的比较

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Cellular interleukin-10 (IL-10) gene from the peripheral blood mononuclear cells of the healthy Dromedary camel (Camelus dromedarius) and viral IL-10 (vIL-10) from the skin scabs of the Dromedary camels infected with contagious ecthyma (a parapoxviral infection in the camels) were amplified by polymerase chain reaction, cloned and characterized. Sequence analysis revealed that the open reading frame (ORF) of dromedarian camel IL-10 is 537. bp in length, encoding 178 amino acid polypeptide while open reading frame of vIL-10 from camel is 561. bp, encoding 187 amino acid polypeptide. The Dromedary camel IL-10 exhibited 62.6% and 68.5% sequence identity at the nucleotide and amino acid level, respectively, with vIL-10 from camel. Sequence analysis also revealed that the Dromedary camel IL-10 shared 99.4% and 98.3% identity at the nucleotide and amino acid level, respectively, with the Bactrian camel (Camelus bactrianus). But vIL-10 from camel shared 84.7% and 83.4% sequence identity at the nucleotide and amino acid level, respectively, with vIL-10 from reindeer (Rangifer tarandus), which is a ruminant species belonging to the order Artiodactyla. The present study was conducted to evaluate the evolutionary origin of the camel parapoxvirus with parapoxviruses of cattle and sheep and the resultant sequence analysis revealed that camel parapoxvirus is closely related to cattle parapoxvirus than sheep parapoxvirus (Orf virus). ? 2012 Elsevier Ltd.
机译：来自健康单峰骆驼（Camelus dromedarius）外周血单核细胞的细胞白介素10（IL-10）基因和来自被传染性疮（副痘病毒）感染的单峰骆驼皮肤sc疮的病毒IL-10（vIL-10）通过聚合酶链反应扩增，克隆和鉴定骆驼感染。序列分析表明，单峰骆驼IL-10的开放阅读框（ORF）长为537. bp，编码178个氨基酸多肽，而骆驼vIL-10的开放阅读框为561. bp，编码187个氨基酸多肽。单峰骆驼IL-10与骆驼中的vIL-10在核苷酸和氨基酸水平上分别表现出62.6％和68.5％的序列同一性。序列分析还显示，单峰骆驼IL-10与双峰驼（Camelus bactrianus）在核苷酸和氨基酸水平上分别具有99.4％和98.3％的同一性。但是骆驼的vIL-10与来自驯鹿（Rangifer tarandus）的vIL-10在核苷酸和氨基酸水平上分别具有84.7％和83.4％的序列同一性，这是一种属于Artiodactyla的反刍动物。本研究旨在评估牛副绵羊病毒与骆驼副痘病毒的进化起源，所得序列分析表明，骆驼副痘病毒与牛副痘病毒比绵羊副痘病毒（Orf病毒）密切相关。 ？ 2012爱思唯尔有限公司

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