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首页> 外文期刊>Cytokine >Aneuploidy assessed by DNA index influences the effect of iron status on plasma and/or supernatant cytokine levels and progression of cells through the cell cycle in a mouse model
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Aneuploidy assessed by DNA index influences the effect of iron status on plasma and/or supernatant cytokine levels and progression of cells through the cell cycle in a mouse model

机译：通过DNA指数评估的非整倍性会影响铁状态对小鼠模型中血浆和/或上清液细胞因子水平以及整个细胞周期中细胞进程的影响

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Aneuploidy, a condition associated with altered chromosome number, hence DNA index, is frequently seen in many diseases including cancers and affects immunity. Iron, an essential nutrient for humans, modulates the immune function and the proliferation of normal and cancer cells. To determine whether impaired immunity seen in iron-deficient subjects may be related to aneuploidy, we measured spleen cell DNA index, percent of cells in different phases of the cell cycle, plasma and/or supernatant IL-2, IL-10, IL-12, and interferon-gamma in control, pair-fed, iron-deficient, and iron-replete mice (N=20-22/group). The test and control diets differed only in iron content (0.09. mmol/kg versus 0.9. mmol/kg) and were fed for 68. days. Mean levels of hemoglobin and liver iron stores of iron-deficient and iron-replete mice were 40-60% lower than those of control and pair-fed mice (P<. 0.05). Mean plasma levels of IL-10, interferon-gamma and percent of cells in S. +. G2/M phases were lower in mice with than in those without aneuploidy (P<. 0.05). Lowest plasma IL-12 and interferon-gamma concentrations were observed in iron-deficient mice with aneuploidy. Mean percents of cultures with aneuploidy and DNA indexes were higher in iron-deficient and iron-replete than in control and pair-fed mice likely due to delayed cell division (P<. 0.05). Aneuploidy decreased the concentration of IL-2 and interferon-gamma in baseline cultures while it increased that of interferon-gamma in anti-CD3 treated cultures. Aneuploidic indexes negatively correlated with cytokine levels, percents of cells in S. +. G2/M phases and indicators of iron status (P<. 0.05). Although chromosome cytogenetics was not performed, for the first time, we report that increased aneuploidy rate may modulate the immune function during iron-deficiency.
机译：非整倍性是一种与染色体数目改变有关的疾病，因此与DNA指数有关，在包括癌症在内的许多疾病中经常出现，并影响免疫力。铁是人类必需的营养素，可调节正常细胞和癌细胞的免疫功能以及其增殖。为了确定在铁缺乏的受试者中观察到的免疫力降低是否可能与非整倍性有关，我们测量了脾细胞DNA指数，细胞周期不同阶段的细胞百分比，血浆和/或上清液IL-2，IL-10，IL-对照，成对喂养，铁缺乏和铁充足的小鼠（N = 20-22 /组）中的γ-干扰素和干扰素-γ。测试日粮和对照日粮的铁含量仅不同（0.09。mmol / kg对0.9。mmol / kg），喂养68天。缺铁和缺铁的小鼠的平均血红蛋白和肝铁储藏水平比对照组和成对喂养的小鼠低40-60％（P <0.05）。 IL.-10的平均血浆水平，干扰素-γ和S. +中细胞的百分比。有G2 / M期的小鼠比没有非整倍性的小鼠的G2 / M期要低（P <0.05）。在具有非整倍性的铁缺乏小鼠中观察到最低的血浆IL-12和干扰素-γ浓度。缺铁和缺铁的非整倍体和DNA指标培养物的平均百分比高于对照组和成对喂养的小鼠，这可能是由于细胞分裂延迟所致（P <0.05）。非整倍性降低了基线培养物中IL-2和干扰素-γ的浓度，而增加了抗CD3处理培养物中干扰素-γ的浓度。非整倍性指数与细胞因子水平，S。+中细胞百分比负相关。 G2 / M期和铁状态指标（P <0.05）。尽管未进行染色体细胞遗传学研究，但我们首次报道非整倍体率增加可能在铁缺乏时调节免疫功能。

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