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首页> 外文期刊>Cytokine >Evaluation of cytokine and chemokine response elicited by Rv2204c and Rv0753c to detect latent tuberculosis infection
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Evaluation of cytokine and chemokine response elicited by Rv2204c and Rv0753c to detect latent tuberculosis infection

机译：评价Rv2204c和Rv0753c诱发的细胞因子和趋化因子应答以检测潜伏性结核感染

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Latent TB infection (LTBI) is one of the major contributing factors for the high incidence of TB in India that in turn significantly contributes to the pool of active TB. Hence, identification and treatment of LTBI is of utmost importance. Currently, no specific diagnostic test is available for LTBI. Earlier, in our immunoproteomic analysis, we identified Rv2204c and Rv0753c protein-containing fractions induced significantly higher interferon-gamma (IFN-gamma) response in LTBI than in active TB. In this study, we evaluated cytokine and chemokine response against M. tuberculosis antigens for improving LTBI identification. Two M. tb proteins Rv2204c and Rv0753c were cloned, over expressed in E. coli and purified by affinity chromatography. Antigen-specific immune response was evaluated in 39 pulmonary TB patients (PTB) and 35 healthy house-hold contacts (HHC). After whole blood culture for 6 days, the secretion of cytokines and chemokines were quantified in culture supernatants using Enzyme Linked Immune Sorbent Assay (ELISA). Antigen specific cytokines such as interferon gamma (IFN-gamma), interleukin-6 (IL-6), IL-8, IL-12p40 and chemokines like monocyte chemotactic proteins MCP-1, MCP-2 were significantly higher in HHC than PTB. In contrast to other cytokines, tumor necrosis factor-alpha (TNF)-alpha response was significantly increased in PTB compared with HHC. Both Rv2204c and Rv0753c antigen specific IFN-gamma response showed 86% positivity in HHC; whereas in PTB, these antigens showed 18% and 21% positivity respectively. Rv2204c antigen-specific IFN-gamma/TNF-alpha response displayed maximum positivity of 91% in HHC and minimum positivity of 10% (4/39) in PTB. Rv2204c and Rv0753c specific IFN-gamma and IFN-gamma/TNF-alpha responses showed the most promising accuracy in identifying LTBI. (C) 2015 Elsevier Ltd. All rights reserved.
机译：潜伏性结核感染（LTBI）是印度结核高发的主要因素之一，而这反过来又大大增加了活动性结核的数量。因此，LTBI的识别和治疗至关重要。当前，尚无针对LTBI的特定诊断测试。早些时候，在我们的免疫蛋白质组学分析中，我们确定了LTBI中含有Rv2204c和Rv0753c的蛋白质组分诱导的干扰素-γ（IFN-γ）应答明显高于活动性TB。在这项研究中，我们评估了针对结核分枝杆菌抗原的细胞因子和趋化因子反应，以改善LTBI鉴定。克隆了两个结核分枝杆菌蛋白Rv2204c和Rv0753c，在大肠杆菌中过表达，并通过亲和层析纯化。在39例肺结核患者（PTB）和35例健康家庭接触者（HHC）中评估了抗原特异性免疫反应。全血培养6天后，使用酶联免疫吸附测定（ELISA）对培养上清液中的细胞因子和趋化因子的分泌进行定量。 HHC中的抗原特异性细胞因子（如干扰素γ（IFN-γ），白介素6（IL-6），IL-8，IL-12p40）和趋化因子（如单核细胞趋化蛋白MCP-1，MCP-2）在HHC中显着高于PTB。与其他细胞因子相反，与HHC相比，PTB中的肿瘤坏死因子-α（TNF）-α反应显着增加。 Rv2204c和Rv0753c抗原特异性IFN-γ反应在HHC中均显示86％的阳性。而在PTB中，这些抗原分别显示出18％和21％的阳性率。 Rv2204c抗原特异性IFN-γ/TNF-α反应在HHC中显示最大阳性91％，在PTB中显示最小阳性10％（4/39）。 Rv2204c和Rv0753c特异的IFN-γ和IFN-γ/TNF-α反应在鉴定LTBI方面显示出最有希望的准确性。 （C）2015 Elsevier Ltd.保留所有权利。

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