Cloning and expression of feline interleukin 15.

Dean GA; Barger A; Lavoy A

首页> 外文期刊>Cytokine >Cloning and expression of feline interleukin 15.

【24h】

Cloning and expression of feline interleukin 15.

机译：猫白介素15的克隆与表达

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A cDNA encoding feline interleukin 15 (IL15) was cloned from the lymph node of a cat infected with feline infectious peritonitis virus. The cDNA is 486bp in length and encodes a protein of 162 amino acids. Recombinant protein was readily expressed as a GST fusion in Escherichia coli and purified by glutathione affinity chromatography. Expression of recombinant protein in mammalian cells was only accomplished by eliminating the 5' and 3' UTR, replacing the IL15 signal peptide with the tissue plasminogen activator signal peptide, and adding 3' sequence to disrupt presumptive secondary structure of the mRNA. Biologically active feline IL15 was expressed in HEK293T cells and was shown to sustain primary feline lymphocytes, a feline T cell line, and mouse CTLL-2 cells. Proliferation of CTLL-2 cells was induced by the recombinant protein in a dose-dependent manner. Monoclonal and polyclonal antibodies against human IL15 recognized feline IL15 in immunofluorescence and Western blot assays. Additionally, feline IL15 was detectable using a commercially available human IL15 ELISA kit.

机译：从感染了猫传染性腹膜炎病毒的猫的淋巴结中克隆了编码猫白介素15（IL15）的cDNA。 cDNA长486bp，编码162个氨基酸。重组蛋白易于在大肠杆菌中表达为GST融合蛋白，并通过谷胱甘肽亲和层析纯化。重组蛋白在哺乳动物细胞中的表达只能通过消除5'和3'UTR，用组织纤溶酶原激活剂信号肽替代IL15信号肽，并添加3'序列来破坏mRNA的假定二级结构来实现。具有生物学活性的猫IL15在HEK293T细胞中表达，并显示可维持原代猫淋巴细胞，猫T细胞系和小鼠CTLL-2细胞。重组蛋白以剂量依赖的方式诱导CTLL-2细胞的增殖。抗人IL15的单克隆和多克隆抗体在免疫荧光和Western印迹检测中识别出猫IL15。另外，使用市售的人IL15 ELISA试剂盒可检测猫IL15。

著录项

来源
《Cytokine》 |2005年第2期|共7页
作者
Dean GA; Barger A; Lavoy A;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类基础医学;
关键词
Interleukin-15; Feline species; Signal Peptides; Recombinant Proteins; 白细胞介素15; 信号肽类; 重组蛋白质类;

机译：Interleukin-15;Feline species;Signal Peptides;Recombinant Proteins;白细胞介素15;信号肽类;重组蛋白质类;

相似文献

外文文献
中文文献
专利

1. Cloning and expression of feline interleukin 15. [J] . Dean GA, Barger A, Lavoy A Cytokine . 2005,第2期

机译：猫白介素15的克隆与表达
2. Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis Molecular cloning of ion channels in Felis catus that are related to periodic paralyses in man: a contribution to the understanding of the genetic susceptibility to feline neck ventroflexion and paralysis [J] . Denise M. N. Sim?es, Ilda S. Kunii, Magnus R. Dias da Silva, Biology Open . 2014,第9期

机译：与人的周期性麻痹相关的猫属猫离子通道的分子克隆：有助于理解猫的颈腹弯曲和麻痹的遗传易感性与人的周期性麻痹相关的猫科动物离子通道的分子克隆：a有助于理解猫颈部室弯曲和麻痹的遗传易感性与人类周期性麻痹有关的猫属离子通道中的离子通道分子克隆：有助于理解猫颈部室弯曲和麻痹的遗传易感性
3. Molecular Cloning of Feline Lung Resistance-Related Protein (LRP) cDNA and its Expression in a Feline Lymphoma Cell Line and Adriamycin-Resistant Subline [J] . Hajime TSUJIMOTO, Kenjiro FUKUSHIMA, Shinobu MATSUURA, The Journal of Veterinary Medical Science . 2006,第8期

机译：猫肺抗性相关蛋白（LRP）cDNA的分子克隆及其在猫淋巴瘤细胞系和耐阿霉素的亚系中的表达
4. CLONING AND EXPRESSION OF PORCINE INTERLEUKIN-10 GENE [C] . W-S Lan, H-J Qiu, H-L Guo, Congress of the International Pig Veterinary Society . 2002

机译：猪白细胞介素-10基因的克隆和表达
5. Glycosylphosphatidylinositol (GPI) anchored interleukin 2 (IL-2) and interleukin 12 (IL-12) cloning, expression and application in melanoma immunotherapy. [D] . Ji, Jianfei. 2003

机译：糖基磷脂酰肌醇（GPI）锚定的白介素2（IL-2）和白介素12（IL-12）的克隆，表达及其在黑色素瘤免疫治疗中的应用。
6. Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34) [O] . Deborah J. Gow, Valerie Garceau, Clare Pridans, -1

机译：猫集落刺激因子受体（CSF-1R）的克隆表达及菌落刺激因子-1（CSF-1）和白介素34（IL-34）刺激的物种特异性分析
7. Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34) [O] . Gow Deborah J., Garceau Valerie, Pridans Clare, 2013

机译：猫集落刺激因子受体（CSF-1R）的克隆表达及菌落刺激因子-1（CSF-1）和白介素34（IL-34）刺激的物种特异性分析
8. Interleukin-8 Expression in Normal Nasal Epithelium and Its Modulation byInfection with Respiratory Syncytial Virus and Cytokines Tumor Necrosis Factor, Interleukin-1, and Interleukin-6 [R] . Becker, S., Koren, H. S., Henke, D. C. 1993

机译：白细胞介素-8在正常鼻上皮细胞中的表达及其对呼吸道合胞病毒和细胞因子肿瘤坏死因子，白细胞介素-1和白细胞介素-6感染的调节作用

Cloning and expression of feline interleukin 15.

摘要

著录项

相似文献

相关主题

期刊订阅