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首页> 外文期刊>Cytokine >Opposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts.
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Opposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts.

机译：bFGF和TGF-β对人牙周膜成纤维细胞胶原代谢的相反作用。

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This study evaluated the effects of bFGF and TGF-beta, individually and combined, on cell proliferation and collagen metabolism. Primary human periodontal ligament cells were stimulated with two concentrations (1 and 10ng/ml) of each growth factor, both individually and combined. Proliferation was determined by a commercial biochemical assay. Real time RT-PCR determined gene expression of MMP-1 and -2, collagen types I and III, TIMP-1, -2 and -3. Autocrine effects on synthesis of bFGF and TGF-beta were evaluated by ELISA. Only TGF-beta, either isolated or associated with bFGF, significantly increased cell proliferation. TGF-beta had anabolic effects, increasing expression of type I and III collagen as well as of TIMPs, whereas bFGF had opposite effects. When bFGF and TGF-beta were associated, the anabolic effects prevailed. Synthesis of TGF-beta was induced only by the association of lower concentrations of the growth factors, whereas there was a dose-dependent production of bFGF. It is concluded that bFGF had a predominantly catabolic effect, and TGF-beta exerted an anabolic effect on hPDL cells.
机译：这项研究分别评估了bFGF和TGF-β对细胞增殖和胶原代谢的影响。分别用两种浓度的每种生长因子（分别为1和10ng / ml）刺激人牙周膜原代细胞。通过商业生化测定确定增殖。实时RT-PCR确定了MMP-1和-2，I和III型胶原，TIMP-1，-2和-3的基因表达。通过ELISA评估自分泌对bFGF和TGF-β合成的影响。只有分离或与bFGF结合的TGF-β可以显着提高细胞增殖。 TGF-β具有合成代谢作用，增加I型和III型胶原以及TIMP的表达，而bFGF具有相反的作用。当bFGF和TGF-β结合时，合成代谢作用占上风。 TGF-β的合成仅通过较低浓度的生长因子的结合来诱导，而bFGF的剂量依赖性产生。结论是，bFGF主要具有分解代谢作用，而TGF-β对hPDL细胞具有合成代谢作用。

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