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首页> 外文期刊>Cytokine >Role of mitogen-activated protein kinases in the iNOS production and cytokine secretion by Salmonella enterica serovar Typhimurium porins.
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Role of mitogen-activated protein kinases in the iNOS production and cytokine secretion by Salmonella enterica serovar Typhimurium porins.

机译：丝裂原活化蛋白激酶在肠炎沙门氏菌血清鼠伤寒沙门氏菌的iNOS产生和细胞因子分泌中的作用。

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摘要

The expression of inducible nitric oxide synthase (iNOS) is a critical factor in both physiological and pathological functions. The present study examined the role of mitogen-activated protein kinases (MAPKs) in the regulation of iNOS and proinflammatory cytokine production in RAW 264.7 cells in response to Salmonella enterica serovar Typhimurium porins. By use of Western blotting for iNOS detection and enzyme-linked immunosorbent assay (ELISA) for quantization of cytokine secretion, selective pharmacological inhibitors of MAPK pathways were tested for dissecting the molecular mechanisms underlying the mediation of these signaling in porins-stimulated murine macrophages. S. enterica serovar Typhimurium porins activated iNOS expression, NO production and interleukin (IL)-6, IL-8 and tumor necrosis factor-alpha (TNF-alpha) release. Treatment of cells with SB203580 and SP600125 (inhibitors of p38 and JNK, respectively) significantly affected porin-stimulated iNOS and NO production. Concomitant decrease inthe proinflammatory cytokine secretion was detected. These data confirm the importance of the MAPKs cascade in macrophage activation by bacterial product opening up new strategies for therapy of septic shock.
机译：诱导型一氧化氮合酶（iNOS）的表达是生理和病理功能中的关键因素。本研究检查了有丝分裂原激活的蛋白激酶（MAPK）在RAW 264.7细胞中对肠炎沙门氏菌血清鼠伤寒沙门氏菌应答的iNOS和促炎细胞因子产生的调节作用。通过使用Western印迹进行iNOS检测和酶联免疫吸附测定（ELISA）量化细胞因子的分泌，测试了MAPK途径的选择性药理抑制剂，以解析在孔蛋白刺激的鼠巨噬细胞中这些信号传导介导的分子机制。肠炎链球菌血清鼠伤寒沙门氏菌可激活iNOS表达，NO产生以及白介素（IL）-6，IL-8和肿瘤坏死因子-α（TNF-α）释放。用SB203580和SP600125（分别是p38和JNK的抑制剂）处理细胞会显着影响孔蛋白刺激的iNOS和NO的产生。同时检测到促炎细胞因子分泌的减少。这些数据证实了MAPK级联在细菌产物开辟治疗败血性休克的新策略方面对巨噬细胞活化的重要性。

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