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首页> 外文期刊>Cytokine >Up-regulation of cell surface Toll-like receptors on circulating gammadelta T-cells following burn injury.
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Up-regulation of cell surface Toll-like receptors on circulating gammadelta T-cells following burn injury.

机译：烧伤后循环γ-T细胞上细胞表面Toll样受体的上调。

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Burn injury is associated with profound inflammation and activation of the innate immune system involving gammadelta T-cells. Similarly, Toll-like receptors (TLR) are associated with activation of the innate immune response; however, it is unclear whether TLR expression is altered in gammadelta T-cells after major burn injury. To study this, male C57BL/6 mice were subjected to burn injury (25% TBSA) and 1 or 7 days thereafter, blood and spleen cells were isolated and subjected to FACs analysis for TLRs and other phenotypic markers (gammadelta TCR, alphabeta TCR, CD69, CD120b). A marked increase in the number of circulating gammadelta T-cells was observed at 24h post-burn (14% vs. 4%) and a higher percentage of these cells expressed TLR-2. TLR-4 expression was also increased post-burn, but to a lesser degree. These changes in TLR expression were not associated with altered CD69 or CD120b expression in gammadelta T-cells. The mobilization of, and increased TLR expression in, gammadelta T-cells was transient, as phenotypic changes were not evident at 7 days post-burn or in gammadelta T-cells from the circulation or spleen. The increases in TLR expression were not observed in alphabeta T-cells after burn injury. In conclusion, 24h after burn injury mobilization of gammadelta T-cells with increased TLR expression was observed. This finding suggests that this unique T-cell population is critical in the innate immune response to injury, possibly through the recognition of danger signals by TLRs.
机译：烧伤与严重的炎症以及涉及γ-T细胞的先天免疫系统的激活有关。类似地，Toll样受体（TLR）与先天免疫应答的激活有关。然而，尚不清楚严重烧伤后γδT细胞中的TLR表达是否改变。为了研究这一点，对雄性C57BL / 6小鼠进行了烧伤（25％TBSA）治疗，此后1或7天，分离了血液和脾细胞，并对其进行了FAC分析，以分析TLR和其他表型标记（γTCR，字母TCR， CD69，CD120b）。在烧伤后24h观察到循环中的γ-T细胞数量显着增加（14％比4％），这些细胞中表达TLR-2的百分比更高。烧伤后TLR-4表达也增加，但程度较小。 TLR表达的这些变化与γδT细胞中改变的CD69或CD120b表达无关。 γδT细胞的动员和TLR表达的增加是短暂的，因为烧伤后7天或来自循环或脾脏的γδT细胞的表型变化不明显。烧伤后未在字母T细胞中观察到TLR表达的增加。总之，观察到烧伤后24小时动员了具有增加的TLR表达的Gammadelta T细胞。这一发现表明，这种独特的T细胞种群可能是通过TLR识别危险信号而在对损伤的天然免疫反应中起关键作用。

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